Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 149 results for: arpin

Showing: 1 - 40 of 149 results

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin

Plasmid

#195092

Purpose

Gene Overexpression of Human SHARPIN

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_DARPinOFF7

Plasmid

#182396

Purpose

Bacterial expression of DARPinOFF7

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

DARPinOFF7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-E01DARPin

Plasmid

#198287

Purpose

Produces PVCs retargeted with anti-EGFR DARPin E01

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-E01DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-CD4DARPin

Plasmid

#198294

Purpose

Produces PVCs retargeted with anti-CD4 DARPin D27.2

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-CD4DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-CD11bDARPin

Plasmid

#198295

Purpose

Produces PVCs retargeted with anti-CD11b DARPin F7

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-CD11bDARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-DARPinOFF7

Plasmid

#182398

Purpose

Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-DARPinOFF7

Tags

Vesicle Nucleating peptide 6 (VNp6)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-A4DARPin

Plasmid

#198288

Purpose

Produces PVCs retargeted with anti-lysozyme DARPin A4

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-A4DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-DARPinOFF7

Plasmid

#182399

Purpose

Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-DARPinOFF7

Tags

Vesicle Nucleating peptide 15 (VNp15)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S178A

Plasmid

#195097

Purpose

Gene Overexpression of Human SHARPINS178A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

S178A

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S131A

Plasmid

#195095

Purpose

Gene Overexpression of Human SHARPINS131A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

S131A

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S146E

Plasmid

#195099

Purpose

Gene Overexpression of Human SHARPIN S146E

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

S146E

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S285A

Plasmid

#195098

Purpose

Gene Overexpression of Human SHARPINS285A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

S285A

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin S146A

Plasmid

#195096

Purpose

Gene Overexpression of Human SHARPINS146A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

S146A

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-SHARPIN L276A

Plasmid

#195093

Purpose

Gene Overexpression of Human SHARPIN L276A

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Mutation

L276A

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-DARPinOFF7

Plasmid

#182397

Purpose

Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DARPinOFF7

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-DARPin-SpyCatcher

Plasmid

#243754

Purpose

For affinity purification of E01 anti-EGFR DARPin fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

DARPin-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQE30-DARPin-E11

Plasmid

#226784

Purpose

Expression of His-tagged DARPin E11 in E. coli

Depositor

Sebastian Mathea

Article

Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

Insert

DARPin E11

Tags

FLAG and His

Expression

Bacterial

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-DARPinOFF7

Plasmid

#182414

Purpose

Bacterial expression of mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-DARPinOFF7

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

Plasmid

#182415

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-DARPinOFF7

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-Darpin E_01

Plasmid

#66996

Purpose

Expresses malE-Darpin E_01 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Darpin E_01

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet

Plasmid

#244039

Purpose

anti mTFP1DARPin_G01 fused to YPet

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet

Plasmid

#244038

Purpose

anti mTFP1DARPin_E11 fused to YPet

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DARPin 1238_G01-mCherry

Plasmid

#244029

Purpose

anti mTFP1DARPin_G01 fused to mCherry

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-mCherry

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-mCherry

Plasmid

#244028

Purpose

anti mTFP1DARPin_E11 fused to mCherry

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-mCherry

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DARPin 1238_E11-YPet-H2B

Plasmid

#244034

Purpose

anchoring anti mTFP1DARPin_E11 fused to YPet to chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet-HIST1H2BJ

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet-CAAX

Plasmid

#244033

Purpose

anchoring anti mTFP1DARPin_G01 fused to YPet to plasma membrane

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet-CAAX

Tags

CAAX

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet-CAAX

Plasmid

#244032

Purpose

anchoring anti mTFP1DARPin_E11 fused to YPet to plasma membrane

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet-CAAX

Tags

CAAX

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DARPin 1238_G01-YPet-H2B

Plasmid

#244035

Purpose

anchoring anti mTFP1DARPin_G01 fused to YPet to chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet-HIST1H2BJ

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-Sharpin NZF (347-376)

Plasmid

#235651

Purpose

Bacterial expression of human Sharpin NZF (347-376)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

Sharpin NZF (SHARPIN Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 347-376 only

Promoter

T7

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_27F3_hFc

Plasmid

#199619

Purpose

Encodes DARPin-hFc 27F3 for mammalian expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-hFc 27F3

Tags

hFc

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_27B3_hFc

Plasmid

#199618

Purpose

Encodes DARPin-hFc 27B3 for mammalian expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-hFc 27B3

Tags

hFc

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQIq_MRGS_HIS8_(27F3)_FLAG

Plasmid

#199615

Purpose

Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27F3

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQIq_MRGS_HIS8_(27G2)_FLAG

Plasmid

#199616

Purpose

Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27G2

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQIq_MRGS_HIS8_( E3_5)_FLAG

Plasmid

#199612

Purpose

Encodes DARPin-FLAG E3_5 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG E3_5 (control)

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQIq_MRGS_HIS8_(27B3)_FLAG

Plasmid

#199613

Purpose

Encodes DARPin-FLAG 27B3 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27B3

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_ E3_5 _hFc

Plasmid

#199617

Purpose

Encodes DARPin-hFc E3_5 for mammalian expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-hFc E3_5 (control)

Tags

hFc

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC0_Empty

Plasmid

#243799

Purpose

pAVEC0 backbone with no fluorophores or tags

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Agrobacterium mediated transformation

Expression

Plant

Promoter

35S

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC0_N-mEGFP

Plasmid

#231255

Purpose

For expressing fusion proteins with N terminally tagged mEGFP in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC0_N-StrepIII

Plasmid

#231260

Purpose

For expressing fusion proteins with N terminal StrepIII tags in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

StrepIII

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1 - 40 of 149 results

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