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Plasmid#81799PurposeGateway Donor vector containing YTHDC1 , part of the Target Accelerator Plasmid Collection.DepositorInsertYTHDC1 (YTHDC1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_YTHDC1_p.E483Q
Plasmid#81551PurposeGateway Donor vector containing YTHDC1 , part of the Target Accelerator Plasmid Collection.DepositorInsertYTHDC1 (YTHDC1 Human)
UseGateway entry vectorTagsExpressionMutationE483QPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-HA-hYTHDC1
Plasmid#85167PurposeExpression of FLAG-HA-tagged human YTHDC1DepositorInsertYTHDC1 (YTHDC1 Human)
UseTagsFlag and HaExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTHDC1-FL
Plasmid#177121PurposeBacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTHDC1 Human)
UseTagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjYTHDC1
Plasmid#122284PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjYTHDC1 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-YTHDC1-FL-mCherry-Cry2
Plasmid#177124PurposeExpress Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet AssayDepositorInsertYTHDC1 (YTHDC1 Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationPromoterSFFVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDC1
Plasmid#228778PurposeProtein expression plasmid for YTH domain of YTHDC1 in E.Coli.DepositorInsertYTH domain of YTHDC1 (YTHDC1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)
Plasmid#102277PurposeFor expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)DepositorInsertYTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
UseFor mammalian cell-free protein expression system…TagsGST, HA, and HISExpressionMutationPromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant
Plasmid#177130PurposeGenerate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1DepositorInsertYTHDC1 (YTHDC1 Human)
UseLentiviralTagsmClover3ExpressionMutationW428A/W377A (m6A binding defects), and siRNA resi…PromoterEf1aAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
4R3H
Plasmid#64652PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertYTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceApril 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR-IDR1-mCherry-Cry2
Plasmid#177125PurposeExpress IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet AssayDepositorInsertYTHDC1 (IDR1 domain) (YTHDC1 Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationIDR1 domain onlyPromoterSFFVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-YTH-mCherry-Cry2
Plasmid#177126PurposeExpress YTH domain fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet AssayDepositorInsertYTHDC1 (YTH domain) (YTHDC1 Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationYTH domain onlyPromoterSFFVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mClover-YTH domain
Plasmid#177123PurposeBacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTH domain) (YTHDC1 Human)
UseTagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialMutationYTH domain only (IDR1 and IDR2 deletion)PromoterT7Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTH-IDR2
Plasmid#177122PurposeBacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
UseTagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialMutationYTH and IDR2 domains only (IDR1 deletion)PromoterT7Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only