We narrowed to 68 results for: 79877

1. eSpCas9(1.1)_No_FLAG

   Plasmid

   #79877

   Purpose

   FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. DCAKD gRNA (BRDN0001144969)

   Plasmid

   #75872

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DCAKD

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DCAKD (DCAKD Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. DCAKD gRNA (BRDN0001148499)

   Plasmid

   #75873

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DCAKD

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DCAKD (DCAKD Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. DCAKD gRNA (BRDN0001148979)

   Plasmid

   #75874

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DCAKD

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DCAKD (DCAKD Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. eSpCas9(1.1)_NoFlag_hROSA26_Left

   Plasmid

   #191442

   Purpose

   Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hROSA26 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223-DCAKD

   Plasmid

   #23585

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   DCAKD (DCAKD Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

6. RhoC FLARE.sc mCer, mVenus - wt

   Plasmid

   #65071

   Purpose

   RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus

   Promoter

   CMV, p10

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

7. RhoC FLARE.sc mCer, mVenus - CA

   Plasmid

   #65424

   Purpose

   RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, Q63L in RhoC

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

8. RhoC FLARE.sc mCer, mVenus - DN

   Plasmid

   #65423

   Purpose

   RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, T19N in RhoC

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-L7-6-Cre

   Plasmid

   #235151

   Purpose

   Expresses Cre recombinase under the Purkinje cell-specific L7-6 promoter

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   Cre
   

   Use

   AAV

   Promoter

   L7-6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET23a-His-TEV-SpyCatcher3

    Plasmid

    #235149

    Purpose

    Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    SpyCatcher3
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-L7-6-DD-T6B-WT-YFP

    Plasmid

    #235145

    Purpose

    Expresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    DHFR-fused T6B peptide
    

    Use

    AAV

    Promoter

    L7-6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-L7-6-FLAG-HA-T6B-WT-YFP

    Plasmid

    #235141

    Purpose

    Expresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    T6B peptide
    

    Use

    AAV

    Tags

    FLAG/HA

    Promoter

    L7-6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV[FLEX]-CAG-EGFP-Ctrl-sponge

    Plasmid

    #235152

    Purpose

    Expresses a EGFP-based control sponge in a Cre recombinase-dependent manner

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    EGFP/control sponge
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET23a-His-TEV-HaloTag-linker-SpyCatcher3

    Plasmid

    #235150

    Purpose

    Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    HaloTag linked to SpyCatcher3
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-DD-T6B-WT-YFP

    Plasmid

    #235143

    Purpose

    Expresses the inducible T6B peptide fused with DHFR and YFP under the human synapsin promoter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    DHFR-fused T6B peptide
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-hSyn-FLAG-HA-T6B-WT-YFP

    Plasmid

    #235139

    Purpose

    Expresses the T6B peptide fused with YFP under the human synapsin promoter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    T6B peptide
    

    Use

    AAV

    Tags

    FLAG/HA

    Promoter

    hSyn

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-L7-6-DD-T6B-MUT-YFP

    Plasmid

    #235146

    Purpose

    Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    DHFR-fused mutant T6B peptide
    

    Use

    AAV

    Mutation

    5 tryptophan residues of T6B-WT mutated

    Promoter

    L7-6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV[FLEX]-CAG-EGFP-pri-miR-Ctrl

    Plasmid

    #235158

    Purpose

    Expresses EGFP and a control primary transcript in a Cre recombinase-dependent manner

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    EGFP/primary transcript with control siRNA
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV[FLEX]-CAG-EGFP-miR-206-sponge

    Plasmid

    #235154

    Purpose

    Expresses a EGFP-based sponge for miR-206 in a Cre recombinase-dependent manner

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    EGFP/miR-206 sponge with 12x binding sites
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge