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pFOSGE_COF3F06

Plasmid

#70920

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc4 (Orc4 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1C09

Plasmid

#70834

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MRG15 (MRG15 Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1E08

Plasmid

#70829

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Cdk9 (Cdk9 Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1G09

Plasmid

#70837

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

TfIIEalpha (TfIIEalpha Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4D02

Plasmid

#71023

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

mof (mof Fly)

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1E04

Plasmid

#70800

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

asf1 (asf1 Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2F08

Plasmid

#70914

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Ssrp (Ssrp Fly)

Use

Gateway entry vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
POSV801-SP44-SnaAinBCDEOT1T2

Plasmid

#225056

Purpose

Heterologous expression in Streptomyces with inactive SnaA

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaA

Expression

Bacterial

Mutation

S556A/S1616A

Promoter

SP44

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POSV801-SP44-SnaABCDEinOT1T2

Plasmid

#225057

Purpose

Heterologous expression in Streptomyces with inactive SnaE

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaE

Expression

Bacterial

Mutation

deltaA46-V801

Promoter

SP44

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
2M-T-Mce3R_K262A

Plasmid

#225728

Purpose

To express Mce3R mutant K262A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Mutation

K262A

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-MelG

Plasmid

#225729

Purpose

To express MelG (Rv1937) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melG

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-VapBC35

Plasmid

#225733

Purpose

To express VapBC35(Rv1962) Toxin antitoxin in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

vapBC35

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
2M-T_Mce3R

Plasmid

#225726

Purpose

To express Mce3R in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
2M-T-Mce3R_R53A

Plasmid

#225727

Purpose

To express Mce3R mutant R53A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R53A

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-TrxC

Plasmid

#225742

Purpose

To express TrxC(Rv3914) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxC

Tags

6xHis

Expression

Bacterial

Mutation

TrxC

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-TrxB2

Plasmid

#225741

Purpose

To express TrxB2(Rv3913) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxB2

Tags

6xHis

Expression

Bacterial

Mutation

TrxB2

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-MelI

Plasmid

#225730

Purpose

To express MelI (Rv1939) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melI

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-MelK

Plasmid

#225732

Purpose

To express MelK (Rv1941) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melK

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-MelJ

Plasmid

#225731

Purpose

To express MelJ (Rv1940) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melJ

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POSV801-SP44-SnaABCDEOinT1T2

Plasmid

#225058

Purpose

Heterologous expression in Streptomyces with inactive SnaO

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaO

Expression

Bacterial

Mutation

deltaR51-V511

Promoter

SP44

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POSV801-SP44-SnaABCDEOT1T2

Plasmid

#225055

Purpose

Heterologous expression in Streptomyces for threopeptin biosynthetic gene cluster

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Expression

Bacterial

Mutation

WT

Promoter

SP44

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSF-coSnaA-TEV-His_His-TEV-SnaC

Plasmid

#225059

Purpose

recombinant expression of SnaA in E. coli

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ChmN-C47A

Plasmid

#221367

Purpose

Express C47A ChmN in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmN-C47A

Tags

His6

Expression

Bacterial

Mutation

changed Cys 47 to Ala

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-degFusionRed

Plasmid

#199463

Purpose

Arabinose-induced expression of the photodegradable His-tagged protein degFusionRed in E. coli.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

degFusionRed

Tags

6xHis

Expression

Bacterial

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Gal4-degFusionRed-p65

Plasmid

#199464

Purpose

Expression of the synthetic photodegradable transcription factor Gal4-degFusionRed-p65 in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

Gal4-degFusionRed-p65

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM634

Plasmid

#134219

Purpose

Baculovirus expression of CENP-HIKM

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP HIKM

Expression

Insect

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
pKM672

Plasmid

#134221

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 1-509

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM692

Plasmid

#134224

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 235-509

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM673

Plasmid

#134222

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 510-end

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-M3C

Plasmid

#107087

Purpose

M. sedula PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3 fused to CyPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15T-MsePCNA2

Plasmid

#107064

Purpose

Expresses M. sedula PCNA2 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15T-MsePCNA1

Plasmid

#107063

Purpose

Expresses M. sedula PCNA1 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-S3C

Plasmid

#107090

Purpose

S. solfataricus PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3 fused to CyPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-S1C

Plasmid

#107088

Purpose

S. solfataricus PCNA1 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA1 fused to CyPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-YS3

Plasmid

#107084

Purpose

S. solfataricus PCNA3 fused at the C-terminus of YPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3 fused to YPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-YS1

Plasmid

#107082

Purpose

S. solfataricus PCNA1 fused at the C-terminus of YPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA1 fused to YPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-M2C

Plasmid

#107086

Purpose

M. sedula PCNA2 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2 fused to CyPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-M1C

Plasmid

#107085

Purpose

M. sedula PCNA1 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1 fused to CyPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-YM3

Plasmid

#107081

Purpose

M. sedula PCNA3 fused at the C-terminus of YPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3 fused to YPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-YM2

Plasmid

#107080

Purpose

M. sedula PCNA2 fused at the C-terminus of YPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2 fused to YPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-YM1

Plasmid

#107079

Purpose

M. sedula PCNA1 fused at the C-terminus of YPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1 fused to YPet

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15T-MsePCNA3

Plasmid

#107065

Purpose

Expresses M. sedula PCNA3 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJExpress414_GmPOPB-R663K

Plasmid

#92239

Purpose

Express R663K mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663K mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Lysine

Promoter

T7 promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJExpress414_GmPOPB-W695del

Plasmid

#92241

Purpose

Express W695-deletion mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-W695deletion mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Tryptophan 695 deleted

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJExpress414_GmPOPB-R663Q

Plasmid

#92240

Purpose

Express R663Q mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663Q mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Glutamine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJExpress414_GmPOPB-H698A

Plasmid

#92237

Purpose

Express H698A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJExpress414_GmPOPB-R663A

Plasmid

#92238

Purpose

Express R663A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFOSGE_COF3C01

Plasmid

#70944

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc1 (Orc1 Fly)

Use

Gateway entry vector

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3F01

Plasmid

#70947

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

ine (ine Fly)

Use

Gateway entry vector

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2E06

Plasmid

#70899

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

NAAT1 (NAAT1 Fly)

Use

Gateway entry vector

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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