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Plasmid#177021PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsertgeraniol synthase
UseSynthetic BiologyTagsExpressionMutationtransit peptide removedPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1G108CP20X
Plasmid#85096PurposeExpresses PCNA1 variant (G108C) fused to putidaredoxin in E. coliDepositorInsertThe G108C variant of PCNA1 fused to putidaredoxin
UseTagsHis6 tagExpressionBacterialMutationG108C in PCNA1PromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-C/pCMV-14-FLAG (Q391-END)
Plasmid#73590PurposeExpress C-terminal part (Q391- end) of human TRAFD1(FLN29) in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-Mid/pCMV-14-FLAG (L175-D390)
Plasmid#73589PurposeExpress middle part (L175-D390) of human TRAFD1 in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-N/pCMV-14-FLAG (1-I210)
Plasmid#73588PurposeExpress N-terminal part (1-I210) of hTRAFD1 (FLN29) in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJH-MBP-CbAgo
Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
UseTagsMPBExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBR15.2-2H12.D11opt
Plasmid#221185PurposecdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)DepositorInsertcdGreen2.1
UseTagsExpressionBacterialMutationPromoterConstitutiveAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pConRef-2H12
Plasmid#221182PurposecdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)DepositorInsertscdGreen
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7·X-nia
Plasmid#122592PurposePlasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion siteDepositorInsertxylS/Pm->Nia
UseSynthetic Biology; Tn7 genomic integrationTagsExpressionBacterialMutationPromoterPmAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPQD2KN0908
Plasmid#177088PurposeMoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB)DepositorInsertP19, CrDXS2, PaGPPS1, and CrGES
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD2KN0897
Plasmid#177090PurposeMoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatBDepositorInsertCrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0770
Plasmid#177060PurposeMoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertloganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0121
Plasmid#177053PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoterDepositorInsertiridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0117
Plasmid#177049PurposeMoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoterDepositorInsertiridoid synthase (CrISY) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0109
Plasmid#177042PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertgeranyl pyrophosphate synthase (PaGPPS1) from Picea abies
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXylA-RgTALsyn
Plasmid#100948PurposeExpression of Tyrosine Ammonia Lyase from constitutive XylA promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterXylA (Constitutive)Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0108
Plasmid#177041PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoterDepositorInsertgeranyl pyrophosphate synthase (PaGPPS1) from Picea abies
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0817
Plasmid#177039PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoterDepositorInserttruncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0119
Plasmid#177051PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein+H34like (NmMLPL) from Nepeta mussinii driven by 35S promoterDepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-N/pIRES puro Glue (M1-A497)
Plasmid#73454PurposeExpress human N-terminal part (M1-A497) Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsCBP tag, Ha tag, and strep tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only