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We narrowed to 4,942 results for: codon optimized

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Showing: 181 - 200 of 4942 results

  2. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid
    #114089
    Purpose
    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized FnCas12a
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114089-plasmid-map-sequence-id-220552

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  3. dCas12Max-CBE

    Plasmid
    #195337
    Purpose
    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon-optimized dCas12Max-CBE
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195337-plasmid-map-sequence-id-383131

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  4. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

    Plasmid
    #133790
    Purpose
    CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized NmeCas9
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CAG
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133790-plasmid-map-sequence-id-268572

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  14. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

    Plasmid
    #114090
    Purpose
    CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized MbCas12a
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    114090-plasmid-map-sequence-id-230476

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  15. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

    Plasmid
    #170273
    Purpose
    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.
    Depositor
    Vladislav Verkhusha
    Publication
    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    See Comments
    Promoter
    CMVd1
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170273-plasmid-map-sequence-id-334945

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  18. MSP2443

    Plasmid
    #72252
    Purpose
    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72252-plasmid-map-sequence-id-207329

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Showing: 181 - 200 of 4942 results