We narrowed to 220 results for: lifeact

1. pDONR221-Lifeact- mScarlet-HA (JDW 1247)

   Plasmid

   #224538

   Purpose

   A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lifeact-mScarlet-HA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-syn-LifeAct-jGCaMP8f-WPRE

   Plasmid

   #186038

   Purpose

   AAV-mediated expression of LifeAct-jGCaMP8f under the synapsin promoter

   Depositor

   Loren Looger

   Insert

   LifeAct-jGCaMP8f
   

   Use

   AAV

   Tags

   LifeAct-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-syn-LifeAct-jGCaMP8s-WPRE

   Plasmid

   #186040

   Purpose

   AAV-mediated expression of LifeAct-jGCaMP8s under the synapsin promoter

   Depositor

   Loren Looger

   Insert

   LifeAct-jGCaMP8s
   

   Use

   AAV

   Tags

   LifeAct-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDAX-EMMI-039 LifeAct(D-G)//Esp3I

   Plasmid

   #130241

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   LifeAct
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-LifeAct-FRB-DMVF-181-N

   Plasmid

   #87610

   Purpose

   FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   LifeAct-FRB-DMVF181-N
   

   Use

   Tags

   17-aa sequence from Abp140 (aka "Lifeact&quo…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-Lifeact-EGFP-3xHA (JDW 1322)

   Plasmid

   #224494

   Purpose

   Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Liefact-EGFP-3xHA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE

   Plasmid

   #186047

   Purpose

   AAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   LifeAct-jGCaMP8f
   

   Use

   AAV

   Tags

   LifeAct-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-CAG-FLEX-LifeAct-jGCaMP8m-WPRE

   Plasmid

   #186048

   Purpose

   AAV-mediated expression of LifeAct-jGCaMP8m under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   LifeAct-jGCaMP8m
   

   Use

   AAV

   Tags

   LifeAct-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pME-Lifeact-mCherry-3xHA (JDW 1321)

   Plasmid

   #224493

   Purpose

   Gateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lifeact-mCherry-3xHA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LifeAct-mNeonGreen -IRES- Talin head-SpyTag003

    Plasmid

    #210024

    Purpose

    Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    LifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Encephalomyocarditis virus internal ribosome entr…

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CAG-FLEX-LifeAct-jGCaMP8s-WPRE

    Plasmid

    #186049

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8s under the CAG promoter, Cre-dependent expression

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8s
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRDAX-VALENTIA-010 LifeAct-Neptune//BsaI

    Plasmid

    #130191

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    LifeAct-Neptune
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDAX-VALENTIA-027 LifeAct-mVenus//BsaI

    Plasmid

    #130208

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    LifeAct-mVenus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRDAX-VALENTIA-009 LifeAct-mVenus//BsaI

    Plasmid

    #130190

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    LifeAct-mVenus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRDAX-VALENTIA-008 LifeAct-Eos//BsaI

    Plasmid

    #130189

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    LifeAct-Eos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRDAX-VALENTIA-001 LifeAct-Sirius//BsaI

    Plasmid

    #130182

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    LifeAct-Sirius
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

    Plasmid

    #175526

    Purpose

    Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag11 and Tomm20-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

    Plasmid

    #175261

    Purpose

    labelling of microtubule plus end and F-actin structures

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
    

    Use

    Tags

    mCherry (on iLifeact) and mNeonGreen (on EB3)

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    pTub-alpha1

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

    Plasmid

    #229826

    Purpose

    Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet-I
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPI226

    Plasmid

    #113228

    Purpose

    safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-mCherry

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    LifeAct
    

    Use

    Safe haven knock-in plasmid for the act5 locus, l…

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    act5

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge