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Rtn2b-R60f-BFP

Plasmid

#186608

Purpose

Encodes human Reticulon 2 disease mutant R60f fused to BFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 mutant R60f (RTN2 Human)

Use

Tags

BFP

Expression

Mammalian

Mutation

c.178insC (R60f)

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Rtn2b-mNeon-TurboID

Plasmid

#186612

Purpose

Third generation lentiviral vector expressing full-length Reticulon 2 fused to mNeonGreen and V5-TurboID

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-271-mNeon

Plasmid

#186601

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 271 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 271 (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-135-mNeon

Plasmid

#186602

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 135 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 135 (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-60-mNeon

Plasmid

#186603

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 60 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 60 (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET29b-AK1-His

Plasmid

#124135

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Adenylate kinase (AK1 Chicken)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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LCC-ICCG_pET21b

Plasmid

#215408

Purpose

Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

LCC-ICCG

Use

Tags

Expression

Bacterial

Mutation

F243I/D238C/S283C/Y127G

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Klc1-GFP

Plasmid

#186617

Purpose

Encodes Kinesin Light Chain 1 fused to GFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Light Chain 1 (KLC1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET151-D-TOPO-PARP14-SΔK

Plasmid

#216658

Purpose

Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

Depositor

Herwig Schüler

Article

Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

Insert

PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

Deletion starting from Q1390 to D1448 and substit…

Promoter

T7

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-NLS-FRB-VP16

Plasmid

#210515

Purpose

expresses NLS-FRB-VP16 component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

NLS-FRB-VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Ca-LiPPI-CLAPon1.0 (APEX)

Plasmid

#213408

Purpose

Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 1)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

MK2-E5-truncated hlov-TEVcs-APEX(201-250)-K5-HA-HA-APEX(2-200)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-bArrestin2-TevC-P2A-TdTomato

Plasmid

#89869

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

R173H in TdTomato (please see depositor comments …

Promoter

CMV

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

Plasmid

#89872

Purpose

expresses CB1R-iTango component in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

CB1R-V2tail-TevN-BLITz1-TetR-VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

Plasmid

#230989

Purpose

Co-expressing membrane-localized Voltron2 and soma-localized CheRiff.

Depositor

Adam Cohen

Article

Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

Insert

Membrane-localized Voltron2 and soma-localized CheRiff

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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PP_080_pAAV_CAG_DIO-LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

Plasmid

#230990

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

Insert

Cre-dependent, membrane-localized Voltron2 and soma-localized CheRiff

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Rtn2b-R60f-mCherry

Plasmid

#186607

Purpose

Third generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 mutant R60f (RTN2 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

c.178insC (R60f)

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Rtn2b-NTD136-271-mNeon

Plasmid

#186604

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 136 to 271 (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-mCherry-Stim1

Plasmid

#186620

Purpose

Third generation lentiviral vector expressing Stim1-wt fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PatoL-OG241

Plasmid

#72841

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoL

Use

Tags

Expression

Bacterial

Mutation

Promoter

PatoL

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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