Addgene

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HIS-HA-CMYC P53

Plasmid

#233738

Purpose

expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

Depositor

Albert Keung

Article

Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

Insert

HIS-HA-CMYC-p53 (TP53 Human)

Use

Tags

HIS-HA-CMYC

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Trp53_i3-ipUSEPR-TR657

Plasmid

#228929

Purpose

Knockdown of Trp53 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Trp53_i3 (Trp53 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Trp53_i4-ipUSEPR-TR657

Plasmid

#228930

Purpose

Knockdown of Trp53 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Trp53_i4 (Trp53 sequence: GTCGCTACCTACAGCCAGGA, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-Puro-TP53

Plasmid

#227319

Purpose

Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53

Plasmid

#221317

Purpose

Dox-inducible expression of IRSp53 in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-hP53DD (TET)

Plasmid

#214083

Purpose

Mammalian expression tetramerization domain (TET) of human TP53

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

hP53DD (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI TP53 S46D

Plasmid

#213002

Purpose

expresses human P53

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

P53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

S46D

Promoter

cmv

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_WRAP53:1-548

Plasmid

#211232

Purpose

Mammalian expression of full-length human WRAP53. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WRAP53:M1-I548 (WRAP53 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI TP53 S46A

Plasmid

#213001

Purpose

expresses human P53

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

P53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

S46A

Promoter

cmv

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WRAP53:1-548

Plasmid

#211306

Purpose

Mammalian expression of full-length human WRAP53. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WRAP53:M1-I548 (WRAP53 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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H6GB1tevP53(304-393)

Plasmid

#200313

Purpose

bacterial expression of p53 TET-CTD

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53(304-393) (TP53 Human)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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H6GB1tevP53(363-393)

Plasmid

#200314

Purpose

bacterial expression of P53 CTD

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53(363-393) (TP53 Human)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-p53 QM

Plasmid

#196268

Purpose

Mammalian expression of 3xHA tagged p53 L25Q,Q26S,F53Q,F54S

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Trp53 QM (Trp53 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

p53 L25Q,Q26S,F53Q,F54S mutant

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgZfp536#1/Cre

Plasmid

#193248

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Zfp536 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgZfp536#1 (Zfp536 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgZfp536#2/Cre

Plasmid

#193249

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Zfp536 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgZfp536#2 (Zfp536 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTp53#3/Cre

Plasmid

#173622

Purpose

Expresses a Tp53-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Tp53 (Trp53 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-TK-Firefly-cjTP53RK

Plasmid

#122283

Purpose

Lentiviral vector for stable expression of Firefly luciferase.

Depositor

Demián Cazalla

Article

Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

Inserts

Firefly luciferase
cjTP53RK 3'UTR

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-p53mut

Plasmid

#99239

Purpose

Mutant p53 encoded by AS-30D rat hepatoma cell line

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 1997 Sep 5;272(36):22776-80

Insert

p53 (Tp53 Rat)

Use

Tags

Expression

Mammalian

Mutation

Silent mutations in translated protein at Gly (10…

Promoter

CMV immediate/early enhancer/promoter

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSI myc Nup53xl

Plasmid

#86001

Purpose

mammalian expression of myc tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Use

Tags

myc

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (Cow 1-82)

Plasmid

#62062

Purpose

Expression of Cow p53 (residues 1-82) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-82

Promoter

T7

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only