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Plasmid#65024PurposeTpR mini-Tn7 vector, mobilizableDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits
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pCRISPR-mcBEST
Plasmid#209416PurposePlasmid for multiplexed cytosine base editing in streptomycetesDepositorInsertsCodon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterbase editing cassette: PtipA ; sgRNAs: PkasO* an…Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSG019
Plasmid#126502PurposePseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHisDepositorInsertdisaggregase ClpG with 6xHis-tag
UseTagsC-terminal 6xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfp(ASV)C
Plasmid#111617PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE81L-KOFP7
Plasmid#160330PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.DepositorInsertKOFP7
UseTags6xHis tagExpressionBacterialMutationPromoterT5Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG020
Plasmid#126503PurposePseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHisDepositorInsertdisaggregase ClpGgi with 6xHis-tag
UseTagsC-terminal 6xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas1_Cas2/3
Plasmid#89240PurposePlasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1DepositorInsertCas1 and Cas2/3
UseTagsStrep II tag on Cas1ExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfpC
Plasmid#111616PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6K-mini-Tn7T-Gm
Plasmid#65022PurposeGmR mini-Tn7 vector, R6K repliconDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceAug. 17, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15b-Opt13
Plasmid#61698PurposeExpresses PTDH mutant Opt13DepositorInsertOpt13
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFT4.2
Plasmid#208807PurposeExpresses the fluorescent protein phiLOV2.1DepositorInsertphiLOV2.1
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHSG+PTDH
Plasmid#66184Purposephosphite dehydrogenaseDepositorInsertPhosphite Dehydrogenase
UseUnspecifiedTagsExpressionMutationE175A, A176RPromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b-Opt12
Plasmid#61697PurposeExpresses PTDH mutant Opt12DepositorInsertOpt12
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFT9.2
Plasmid#208809PurposeExpresses the fluorescent protein GFPmut2DepositorInsertGFPmut2
UseTagsExpressionMutationPromotertacAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFT3.2
Plasmid#208806PurposeExpresses the fluorescent protein iLOVDepositorInsertiLOV
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQFT
Plasmid#203674PurposepQF derivative with promoter PQ5 exchanged for promoter PQJDepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL2020
Plasmid#86709PurposearaBAD based expression vector for inducible protein production in bacterial hostsDepositorTypeEmpty backboneUseTagsHis10 tagExpressionBacterialMutationPromoteraraBADAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTDH
Plasmid#66183PurposeExpresses phosphite dehydrogenase in E. coliDepositorInsertPhosphite Dehydrogenase
UseTagsHis6 tagExpressionBacterialMutationE175A, A176RPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFT7.2
Plasmid#208808PurposeExpresses the fluorescent protein FASTDepositorInsertFAST
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only