We narrowed to 4,276 results for: GCA

1. eGFP L138 gRNA

   Plasmid

   #119133

   Purpose

   gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   eGFP L138 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   U6

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbdCas9S_P0526-sgRNAmreB

   Plasmid

   #149654

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, P0526

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTorPE-G-GECO1

   Plasmid

   #32466

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.0
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

   Expression

   Bacterial

   Mutation

   GCaMP3 K119I/L173Q/S404G/E430V

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. px458_2A_GFP_sgRNA_TRIM25_G1

   Plasmid

   #127119

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA TRIM25 (TRIM25 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuidPuro-hTP63_sgRNA-1

   Plasmid

   #88854

   Purpose

   CRISPR KO of Trp63

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp63
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTX1-SMN1-exon7-ESE1

   Plasmid

   #126041

   Purpose

   In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

   Depositor

   Frédéric Allain

   Article

   Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

   Insert

   SMN1 exon7 ESE1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX335-NEAT1_IS_v1

   Plasmid

   #97085

   Purpose

   Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1_IS_v1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-PpU6P-sgRNA-L5L2

   Plasmid

   #113738

   Purpose

   Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   P. patens U6 promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROP_C9_Puro_PARP1

   Plasmid

   #183312

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   PARP1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-PpU6P-sgRNA-R4R3

    Plasmid

    #113739

    Purpose

    Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-PpU6P-sgRNA-L5L4

    Plasmid

    #113741

    Purpose

    Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-PpU6P-sgRNA-L1R5

    Plasmid

    #113737

    Purpose

    Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TetO(3G)-WPRE

    Plasmid

    #186206

    Purpose

    tTA dependent gene expression vector

    Depositor

    Naoshige Uchida

    Article

    Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TetO(3G)

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-PpU6P-sgRNA-L1L2

    Plasmid

    #113735

    Purpose

    Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLSU4cg10

    Plasmid

    #126078

    Purpose

    Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    longCaMV35S

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR_eGFP_191

    Plasmid

    #111821

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 171-191
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJFRC7-UAS-NPRR_ANP

    Plasmid

    #133243

    Purpose

    Fluorescent reporter for neuropeptide release imaging

    Depositor

    David Anderson

    Article

    Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

    Insert

    rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pColE1_sgRNA_M1NS_AmpR

    Plasmid

    #119155

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROP_C9_Puro_IDH2

    Plasmid

    #183301

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets IDH2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    IDH2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-Control-PTEN-3'UTR-Mut

    Plasmid

    #21327

    Depositor

    Joshua Mendell

    Article

    O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

    Insert

    PTEN 3' UTR Mutant (PTEN Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    2 miR binding site mutated. Changed from TTTGCAC …

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only