We narrowed to 4,010 results for: SPL

Showing: 1981 - 2000 of 4010 results

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GST-N1IC

Plasmid

#47612

Purpose

bacterial expression of GST-myc-Notch1 IC

Depositor

Urban Lendahl

Article

Beatus et al Development. 1999 Sep;126(17):3925-35.

Insert

Notch1 intracellular domain (Notch1 Mouse)

Tags

GST and myc

Expression

Bacterial

Mutation

contains amino acids 1753-2531

Promoter

tac

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

Plasmid

#140226

Purpose

Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

Luciferase + crIFNg

Expression

Mammalian

Promoter

TRE3G

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET:GACT:GFP

Plasmid

#60734

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3-ySet2

Plasmid

#51698

Purpose

Expresses full length yeast Set 2 gene in a mamalian expression vector

Depositor

Tom Misteli

Article

Luco et al Science. 2010 Feb 19;327(5968):996-1000.

Insert

yeast Set2 (SET2 Budding Yeast)

Tags

C-terminal FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP alpha isoform

Plasmid

#22146

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pSC-SmBiT

Plasmid

#207632

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-SmBiT

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I gamma

Plasmid

#22156

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Dec. 9, 2009

Availability

Academic Institutions and Nonprofits only
PEP113E

Plasmid

#97399

Purpose

binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Peroxisome target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEBB-II-I-GFP gamma isoform

Plasmid

#22148

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pU6_(GLuc)_INT[Spinach2]

Plasmid

#68430

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing the "Spinach2" aptamer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_TOP2

Plasmid

#68435

Purpose

Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP2 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pSiMPlh_N + pSiMPlh_C

Plasmid

#134316

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

hygR_gp41-1 N-intein + hygR_gp41-1 C-intein

Expression

Bacterial

Mutation

P254Q in hygR_gp41-1 N-intein

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pB-bsd-Flag-Trl1

Plasmid

#126609

Purpose

Expresses N-terminal Flag-tagged yeast Trl1

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

tRNA ligase 1 (TRL1 Budding Yeast)

Tags

Flag

Expression

Mammalian

Promoter

CAG

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I alpha

Plasmid

#22154

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Flag-Timeless

Plasmid

#22892

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Timeless (TIMELESS Human)

Tags

Flag and His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP beta isoform

Plasmid

#22147

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
SNV-gfp-IRES-MCS

Plasmid

#53122

Purpose

Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

Depositor

Jeanette Hyer

Article

Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

Type

Empty backbone

Use

Retroviral; For expression in chicken cells

Tags

EGFP-IRES

Promoter

SNV (spleen necrosis virus)

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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cPE

Plasmid

#183549

Purpose

Expresses compact Prime Editor in mammalian cells

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

SpCas9 nickase (H840A), Reverse Transcriptase

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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RFP-FKBP-U2AF35

Plasmid

#187969

Purpose

expresses U2AF35 with N-terminal tags RFP and FKBP

Depositor

Markus Wahl

Article

Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

Insert

U2AF35 (U2af1 Mouse)

Use

Cmv promoter

Tags

mRFP-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MyD88-NpLxIS

Plasmid

#131347

Purpose

Expresses MyD88 with 2 copies of pLxIS motif from STING fused to its N-ternimus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88-NpLxIS (Myd88 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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