We narrowed to 5,822 results for: pcas

1. pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

   Plasmid

   #148857

   Purpose

   Mammalian Expression of HsPatL1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsPatL1-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

   Plasmid

   #148858

   Purpose

   Mammalian Expression of HsTNRc6C-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRc6C-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAS-mak10 gRNA-MAK10Δ

   Plasmid

   #160384

   Purpose

   For CRISPR-mediated deletin og MAK10 in yeast

   Depositor

   John McCusker

   Insert

   MAK10
   

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXD70LacZ8-CadR-Pcadh-LacZ

   Plasmid

   #191623

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadR

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Tesmin-BioID2-3xFLAG

   Plasmid

   #186815

   Purpose

   CAG promoter-driven expression of TESMIN-BioID2-3xFLAG

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Insert

   Tesmin (Tesmin Mouse)
   

   Tags

   BioID2-3xFLAG

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-MBP-ATG9-Q835A

   Plasmid

   #188081

   Purpose

   Expresses human ATG9-Q835A mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   Q835A

   Promoter

   CMV

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-MBP-ATG9-K838A

   Plasmid

   #188078

   Purpose

   Expresses human ATG9-K838A mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   K838A

   Promoter

   CMV

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-MBP-ATG9-V839A

   Plasmid

   #188077

   Purpose

   Expresses human ATG9-V839A mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   V839A

   Promoter

   CMV

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCALNL-tdTomato-2A-mSyp::EGFP

   Plasmid

   #191213

   Purpose

   Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

   Depositor

   Theofanis Karayannis

   Article

   Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

   Insert

   tdTomato-T2A-mSyp::EGFP (Syp Mouse)
   

   Use

   Pcagen

   Tags

   mSyp::EGFP

   Expression

   Mammalian

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-WIPI2d (FRRG/AAAA)-TS

    Plasmid

    #190037

    Purpose

    Expression of wipi2d mutant

    Depositor

    James Hurley

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    TEV-2xStrep tag

    Expression

    Mammalian

    Mutation

    223FRRG to AAAA

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2xNLS-NLP-cMyc AspCas12a

    Plasmid

    #182120

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc AspCas12a
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-MBP-ATG9-L832A

    Plasmid

    #188084

    Purpose

    Expresses human ATG9-L832A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    L832A

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-MBP-ATG9-V836A

    Plasmid

    #188080

    Purpose

    Expresses human ATG9-V836A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    V836A

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-MBP-ATG9-H837A

    Plasmid

    #188079

    Purpose

    Expresses human ATG9-H837A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    H837A

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-MBP-ATG9 (1-830)

    Plasmid

    #188086

    Purpose

    Expresses human ATG9 C-terminal truncation mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (1-830)

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-MBP-ATG9-P833A

    Plasmid

    #188083

    Purpose

    Expresses human ATG9-P833A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    P833A

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-GST-ATG101-Y45D

    Plasmid

    #188105

    Purpose

    Expresses human ATG101 HORMA Y45D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG101 (ATG101 Human)
    

    Tags

    GST tag

    Expression

    Mammalian

    Mutation

    codon-optimized ATG101-Y45D

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-eDHFR(69K6)-iRFP713

    Plasmid

    #178855

    Purpose

    Mammalian expression of eDHFR(69K6)-iRFP713

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    eDHFR(69K6)-iRFP713
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCasper-pNos-MCP-mCherry

    Plasmid

    #180275

    Purpose

    to epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)

    Depositor

    Hernan Garcia

    Article

    Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

    Insert

    MCP-mCherry
    

    Tags

    mCherry

    Expression

    Insect

    Promoter

    nanos

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid

    #179528

    Purpose

    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 R1333A/R1335A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginines 1333 and 1335 to alanine

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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