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pFRT_TO_FlagHA_G3BP1

Plasmid

#127106

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP1 (G3BP1 Human)

Expression

Mammalian

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 T18N

Plasmid

#47890

Purpose

Expresses myc tagged Miro1 T18N dominant negative mutant

Depositor

Pontus Aspenström

Publication

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 T18N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-Voltron-ST

Plasmid

#119034

Purpose

Express soma-localized Voltron in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Voltron-ST

Expression

Mammalian

Promoter

CAG

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-BTK

Plasmid

#23918

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BTK (BTK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pKM512

Plasmid

#140192

Purpose

For curing of Orbit-integrated plasmids and associated drug marker

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

zeocin resistance gene
Bxb1gp47 (directionality factor)

Expression

Bacterial

Mutation

Cloned in gp47 directinality factor in place of R…

Promoter

EM7 and Ptet

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-PinkyCaMP

Plasmid

#232856

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under hSyn promoter

Depositor

Olivia Masseck

Publication

Fink et al Nat Methods. 2026 Apr 24. doi: 10.1038/s

Insert

PinkyCaMP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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ERroGFP_iE_pCDNA3

Plasmid

#47954

Purpose

Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged redox probe roGFPiE (roGFP C147, E147b, C204)

Depositor

David Ron

Publication

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERroGFP

Tags

FLAG

Expression

Mammalian

Mutation

insertion E147B

Available since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-CSK

Plasmid

#23941

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSK (CSK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Sirius/pcDNA3

Plasmid

#51957

Purpose

mammalian expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

Depositor

Takeharu Nagai

Publication

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sirius

Expression

Mammalian

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAHYB-GFP

Plasmid

#85074

Purpose

Expression of GFP in Pichia pastoris

Depositor

Sheng Yang

Publication

Yang et al Yeast. 2014 Apr;31(4):115-25.

Insert

GFP

Expression

Yeast

Promoter

AOX1

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pB80-hKif5b(1-560)rigor-2xmNeongreen

Plasmid

#174649

Purpose

Live-cell marker for stable microtubules

Depositor

Lukas Kapitein

Publication

Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)

Tags

mNeongreen-mNeongreen

Expression

Mammalian

Mutation

rigor mutation G234A

Promoter

Chicken beta-actin

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hFLT1

Plasmid

#83435

Purpose

human VEGFR1 with HA and FLAG tag

Depositor

Christie Thomas

Publication

Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

Insert

human VEGFR1 (FLT1 Human)

Tags

Flag and Myc

Expression

Mammalian

Mutation

HA-tag was inserted 30 AA downstream of the FLT1 …

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGE010

Plasmid

#71536

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Publication

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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[#GS5-RV] MitoTRACER-Donor

Plasmid

#233505

Purpose

Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

Depositor

Simon Grelet

Publication

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Retroviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMM114

Plasmid

#127211

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, IPT, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pSkylan-NS-N1

Plasmid

#86785

Purpose

for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

Depositor

Pingyong Xu

Publication

Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

Type

Empty backbone

Tags

Skylan-NS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA-FLAG-METTL3-APPA

Plasmid

#160251

Purpose

Express catalytic inactive FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Publication

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGWB555

Plasmid

#74885

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mRuby3-18aa-actin

Plasmid

#74255

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to actin

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

actin

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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