2,294 results

1. PH-mCherry-FRBx2

   Plasmid

   #193570

   Purpose

   Expresses PH domain from PLC delta1 tagged with mCherry and two FRB mutants (T2098L) in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   PH domain from PLC delta1 (Plcd1 Rat)
   

   Tags

   FRB (T2098L) and mCherry

   Expression

   Mammalian

   Mutation

   PH domain

   Promoter

   CMV

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-fDIO-mScarlet-Gphn_C4a

   Plasmid

   #194973

   Purpose

   AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4a) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mScarlet-Gphn (isoform C4a) (Gphn Synthetic, Rat)
   

   Use

   AAV

   Tags

   mScarlet

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-fDIO-mScarlet-Gphn_C4d

   Plasmid

   #194975

   Purpose

   AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4d) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mScarlet-Gphn (isoform C4d) (Gphn Synthetic, Rat)
   

   Use

   AAV

   Tags

   mScarlet

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-fDIO-mEGFP-Gphn_P1

   Plasmid

   #194978

   Purpose

   AAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mEGFP-Gphn (isoform P1) (Gphn Synthetic, Rat)
   

   Use

   AAV

   Tags

   mEGFP

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-fDIO-mScarlet-Gphn_P1

   Plasmid

   #194972

   Purpose

   AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform P1) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mScarlet-Gphn (isoform P1) (Gphn Synthetic, Rat)
   

   Use

   AAV

   Tags

   mScarlet

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-fDIO-mScarlet-Gphn_C4c

   Plasmid

   #194974

   Purpose

   AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4c) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mScarlet-Gphn (isoform C4c) (Gphn Synthetic, Rat)
   

   Use

   AAV

   Tags

   mScarlet

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PVALB mAb_BCN14.1.1C9_LC_Rat Ig Kappa

   Plasmid

   #182891

   Purpose

   Mammalian expression of PVALB rat mAb LC gene

   Depositor

   Heng Zhu

   Insert

   anti-PVALB (human) light chain (PVALB Rat)
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Mutation

   No

   Promoter

   CMV

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

   Plasmid

   #182894

   Purpose

   Mammalian expression of PVALB rat mAb HC gene

   Depositor

   Heng Zhu

   Insert

   anti-PVALB (human) heavy chain (PVALB Rat)
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Mutation

   No

   Promoter

   CMV

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

   Plasmid

   #182895

   Purpose

   Mammalian expression of PVALB rat mAb LC gene

   Depositor

   Heng Zhu

   Insert

   anti-PVALB (human) light chain (PVALB Rat)
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Mutation

   No

   Promoter

   CMV

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-7aa-cb5-pEGFP-C1

    Plasmid

    #177407

    Purpose

    To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid

    #190174

    Purpose

    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway

    Depositor

    Gary Rudnick

    Article

    Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.

    Insert

    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    

    Tags

    FLAG, His6, and c-myc

    Expression

    Mammalian

    Mutation

    changed Cys109 to alanine, changed tyr107 to cyst…

    Promoter

    CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_rSERT_X5C_S277C

    Plasmid

    #190177

    Purpose

    For mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibility

    Depositor

    Gary Rudnick

    Article

    Zhang et al J Biol Chem. 2006 Nov 24;281(47):36213-2

    Insert

    rat serotonin transporter (Slc6a4 Rat)
    

    Tags

    FLAG, His6, and c-myc

    Expression

    Mammalian

    Mutation

    Cys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…

    Promoter

    CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1-3x-NLS-mScarlet-AMPKα2

    Plasmid

    #192452

    Purpose

    mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
    

    Tags

    3x Nuclear localization signal (NLS) and mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-mScarlet-AMPKα2

    Plasmid

    #192451

    Purpose

    mScarlet-tagged alpha2 isoform of AMPK catalytic subunit.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    mScarlet-AMPKalpha2 (Prkaa2 Rat)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

    Plasmid

    #190154

    Purpose

    Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp3 (Vamp2 Rat)
    

    Use

    AAV

    Tags

    P2AT2A-EGFP-caax

    Expression

    Mammalian

    Mutation

    Truncation that includes only rat Vamp3 AA #1-81

    Promoter

    MBP

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. RAT +D122H

    Plasmid

    #187426

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Expression

    Insect

    Mutation

    changed aspartic acid at 129 for histidine

    Promoter

    PH and p10

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RAT +R111E

    Plasmid

    #187425

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Expression

    Insect

    Mutation

    changed arginine at 118 to glutamic acid

    Promoter

    PH and p10

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-Myo1b motor

    Plasmid

    #187364

    Purpose

    Expresses rat myosin 1b isoform b motor domain labelled with EGFP

    Depositor

    Evelyne Coudrier

    Article

    Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

    Insert

    myosin 1b isoform b motor domain (Myo1b Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFP-Myo1b-Tail

    Plasmid

    #187365

    Purpose

    Expresses rat myosin 1b isoform b Tail domain labelled with EGFP

    Depositor

    Evelyne Coudrier

    Article

    Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

    Insert

    myosin 1b isoform b Tail domain (Myo1b Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-Myo1-IQ-Tail

    Plasmid

    #187363

    Purpose

    Expresses rat myosin 1b isoform b IQTail domain labelled with EGFP

    Depositor

    Evelyne Coudrier

    Article

    Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

    Insert

    myosin 1b isoform b IQTail domain (Myo1b Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge