2,226 results

1. CBSH3- 3m shRNA2

   Plasmid

   #160211

   Purpose

   Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …

   Promoter

   mouse U6 promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CBSH3- 3m shRNA1

   Plasmid

   #160209

   Purpose

   Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…

   Promoter

   mouse U6 promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CBSH3- shRNA1

   Plasmid

   #160208

   Purpose

   Knock Down MQWSH3- Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat, Human)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine

   Plasmid

   #160069

   Purpose

   Conditionally overexpress collybistin (CB2SH3-) and mCitrine

   Depositor

   Angel de Blas

   Article

   George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   AAV

   Tags

   HA- in CB2SH3

   Expression

   Mutation

   Promoter

   human Synapsin promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. S2 1058 APOBEC3 no UGI

   Plasmid

   #135350

   Purpose

   Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-rAPOBEC3-ID-1058
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. S1 1029 CP APOBEC3 no UGI

   Plasmid

   #135352

   Purpose

   Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   rAPOBEC3-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. S1 1029 AIDx with UGI

   Plasmid

   #135354

   Purpose

   Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   hAIDx-nSpyCas9 aa-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-HisFlag-rUSP19ER-CRmut

   Plasmid

   #155253

   Purpose

   for mammalian overexpression of the mutated ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (ER isoform) CR mutant (Usp19 Rat)
   

   Use

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Cys(545) to Arg

   Promoter

   CMV

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-rUSP19CYT-CAmut

   Plasmid

   #155250

   Purpose

   for mammalian overexpression of the mutated CYT isoform of rat USP19 fused to GFP at N-terminus

   Depositor

   Simon Wing

   Insert

   USP19 (Cytoplasmic isoform) CA mutant (Usp19 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cys(545) to Ala

   Promoter

   CMV

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-rUSP19ER-CAmut

    Plasmid

    #155249

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminus

    Depositor

    Simon Wing

    Insert

    USP19 (ER isoform) CA mutant (Usp19 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-rUSP19CYT

    Plasmid

    #155248

    Purpose

    for mammalian overexpression of the CYT isoform of rat USP19 fused to GFP at N-terminus

    Depositor

    Simon Wing

    Insert

    USP19 (Cytoplasmic isoform) (Usp19 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-rUSP19ER

    Plasmid

    #155247

    Purpose

    for mammalian overexpression of the ER isoform of rat USP19 fused to GFP at N-terminus

    Depositor

    Simon Wing

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-HisFlag-rUSP19ER deltaN3

    Plasmid

    #155243

    Purpose

    for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-HisFlag-rUSP19ER deltaN2

    Plasmid

    #155242

    Purpose

    for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-HisFlag-rUSP19ER deltaN1

    Plasmid

    #155241

    Purpose

    for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (delta1-536) (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-HisFlag-rUSP19CYT

    Plasmid

    #155238

    Purpose

    for mammalian overexpression of the cytoplasmic isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (Cytoplasmic isoform) (Usp19 Rat, Mouse)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCIS-rPALcc

    Plasmid

    #145804

    Purpose

    expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells

    Depositor

    Richard Mains, Betty Eipper

    Article

    Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9

    Insert

    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed serine 767 to alanine to prevent glycosyl…

    Promoter

    CMV

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mTurquoise2-RnBarr2

    Plasmid

    #137798

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mTurquoise2-RnBarr1

    Plasmid

    #137795

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RnAT1aR DRY->AAY-p2A-mCherry

    Plasmid

    #137784

    Purpose

    Visualization of the At1aR DRY mutant

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat At1a receptor; DRY mutant (Agtr1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DRY -> AAY

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge