We narrowed to 62,913 results for: cloning

Showing: 2001 - 2020 of 62913 results

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pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

Plasmid

#132405

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP15

Plasmid

#141268

Purpose

Gateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP8

Plasmid

#141262

Purpose

Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP7

Plasmid

#141261

Purpose

Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pT076

Plasmid

#137880

Purpose

Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

Depositor

Lindy Barrett

Publication

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Expression

Mammalian

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

Plasmid

#113865

Purpose

Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Publication

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-iRFP
PTCH1-mTurquoise2
CBF-tdTomato

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TGFB1_pLX307

Plasmid

#98377

Purpose

Lentiviral expression of TGFB1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pNIC28-DENV3-NS5FL

Plasmid

#86013

Purpose

Expression of dengue virus 3 NS5 protein

Depositor

Dahai Luo

Publication

Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

Insert

DENV3-NS5FL

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

amino acid residues 6 to 895 of DENV3 NS5

Promoter

T7

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBK-YEE-BE3

Plasmid

#85177

Purpose

Expresses YEE-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YEE-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pScarlessHD-sfGFP-DsRed

Plasmid

#80811

Purpose

Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Kate O'Connor-Giles

Insert

sfGFP-piggyBac-DsRed

Use

Unspecified

Tags

sfGFP

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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Talin-CS

Plasmid

#83377

Purpose

Control FRET probe for measuring tension on Talin molecule

Depositor

Martin Schwartz

Publication

Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

Insert

Talin1 (Tln1 Mouse)

Use

Retroviral

Tags

EGFP-F40-tagRFP (tension sensor module)

Expression

Mammalian

Mutation

Tension sensor module is inserted at C-terminal o…

Promoter

CMV

Available since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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MP4

Plasmid

#69652

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Publication

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA

Expression

Bacterial

Promoter

E. coli PBAD

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMM290

Plasmid

#46038

Depositor

Raymond Monnat

Publication

Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

Insert

WRN (WRN Human)

Use

Tre, bidirectional promoter

Tags

5x myc

Expression

Mammalian

Mutation

S2G introduced during cloning

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-EmGFP-LATS2/1 KD

Plasmid

#52085

Purpose

Lentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporter

Depositor

Yutaka Hata

Publication

Yang et al Mol Cell Biol. 2014 Feb 18.

Insert

LATS2 and LATS1 miRNAs (LATS1, LATS2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MSCV-N LMP2A

Plasmid

#37963

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

LMP2A (LMP-2A H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-PFKFB3

Plasmid

#23668

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKFB3 (PFKFB3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEST53-LRRK2-3XKD

Plasmid

#25049

Depositor

Mark Cookson

Publication

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Flag-HA-USPL1

Plasmid

#22607

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USPL1 (USPL1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCG142

Plasmid

#17246

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pie-1 intron:pie-1 promoter in PDONRP4P1R

Use

Gateway Cloning

Available since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST17-UbE2L3

Plasmid

#15795

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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