Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 24,925 results for: crispr

Clear keywords
Showing: 2001 - 2020 of 24925 results

  1. PX378 Sutterella wadsworthensis Cas9

    Plasmid
    #68315
    Purpose
    Sutterella wadsworthensis Cas9
    Depositor
    Feng Zhang
    Insert
    SwCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68315-plasmid-map-sequence-id-119526

    Enlarge

  2. PX387 Mycoplasma mobile Cas9

    Plasmid
    #68324
    Purpose
    Mycoplasma mobile Cas9
    Depositor
    Feng Zhang
    Insert
    MmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68324-plasmid-map-sequence-id-119539

    Enlarge

  3. PX392 Azospirillum B510 Cas9

    Plasmid
    #68329
    Purpose
    Azospirillum B510 Cas9
    Depositor
    Feng Zhang
    Insert
    AzoCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68329-plasmid-map-sequence-id-119551

    Enlarge

  9. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    171991-plasmid-map-sequence-id-338127

    Enlarge

  10. pCMV-HF-BE3

    Plasmid
    #87439
    Purpose
    Expresses HF-BE3 with C-terminal NLS in mammalian cells
    Depositor
    David Liu
    Publication
    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HF-BE3 (Apobec1 Rat, Bacillus phage PBS2, Human)
    Expression
    Mammalian
    Mutation
    Mammalian codon-optimized Cas9-HF
    Promoter
    CMV
    Available since
    June 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    87439-plasmid-map-sequence-id-298693

    Enlarge

  11. hSynapsin-PE2-Cterminal-lenti

    Plasmid
    #135956
    Purpose
    Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Publication
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) C-terminal
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    MMLV RT(D200N, T306K, W313F, T330P, L603W)
    Promoter
    hSynapsin
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    135956-plasmid-map-sequence-id-269513

    Enlarge

  12. pCjedCas9

    Plasmid
    #172216
    Purpose
    Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells
    Depositor
    John van der Oost
    Publication
    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    Tags
    Histag
    Mutation
    changed Asp 8 to Ala and His 559 to Ala
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    172216-plasmid-map-sequence-id-338133

    Enlarge

  13. pMuLE ENTR SV40-hCas9 L3-L2

    Plasmid
    #62133
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized Cas9
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    62133-plasmid-map-sequence-id-101417

    Enlarge

  14. pHS1950

    Plasmid
    #205983
    Purpose
    Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Inserts
    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    CRISPR array
    Tags
    14xHis and TwinStrep-tag
    Expression
    Bacterial
    Promoter
    T7 (proteins), pJ23119 (crRNA)
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205983-plasmid-map-sequence-id-415374

    Enlarge

  16. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Publication
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

    Enlarge

  17. pCas9-beta-mmr

    Plasmid
    #169241
    Purpose
    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations
    Depositor
    Brian Pfleger
    Publication
    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:
    Inserts
    cas9
    beta
    mutL-E36K
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed glutamic acid 36 to lysine
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169241-plasmid-map-sequence-id-333575

    Enlarge

  18. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid
    #52628
    Purpose
    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency
    Depositor
    Scot Wolfe
    Insert
    Kanamycin cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    52628-plasmid-map-sequence-id-217983

    Enlarge

  19. AICSDP-8:TOMM20-mEGFP

    Plasmid
    #87423
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    6 Articles
    87423-plasmid-map-sequence-id-173982

    Enlarge

Showing: 2001 - 2020 of 24925 results