We narrowed to 5,878 results for: pcas

Showing: 2001 - 2020 of 5878 results

Results per page:

Sort results by:

pCAG-MBP-ATG9-Q835A

Plasmid

#188081

Purpose

Expresses human ATG9-Q835A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

Q835A

Promoter

CMV

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-ATG9-K838A

Plasmid

#188078

Purpose

Expresses human ATG9-K838A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

K838A

Promoter

CMV

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-ATG9-V839A

Plasmid

#188077

Purpose

Expresses human ATG9-V839A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

V839A

Promoter

CMV

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCALNL-tdTomato-2A-mSyp::EGFP

Plasmid

#191213

Purpose

Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

Depositor

Theofanis Karayannis

Article

Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

Insert

tdTomato-T2A-mSyp::EGFP (Syp Mouse)

Use

Pcagen

Tags

mSyp::EGFP

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-WIPI2d (FRRG/AAAA)-TS

Plasmid

#190037

Purpose

Expression of wipi2d mutant

Depositor

James Hurley

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

WIPI2d (WIPI2 Human)

Tags

TEV-2xStrep tag

Expression

Mammalian

Mutation

223FRRG to AAAA

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
2xNLS-NLP-cMyc AspCas12a

Plasmid

#182120

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-ATG9-L832A

Plasmid

#188084

Purpose

Expresses human ATG9-L832A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

L832A

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-ATG9-V836A

Plasmid

#188080

Purpose

Expresses human ATG9-V836A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

V836A

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-ATG9-H837A

Plasmid

#188079

Purpose

Expresses human ATG9-H837A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

H837A

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-ATG9 (1-830)

Plasmid

#188086

Purpose

Expresses human ATG9 C-terminal truncation mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (1-830)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MBP-ATG9-P833A

Plasmid

#188083

Purpose

Expresses human ATG9-P833A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

P833A

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-GST-ATG101-Y45D

Plasmid

#188105

Purpose

Expresses human ATG101 HORMA Y45D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG101 (ATG101 Human)

Tags

GST tag

Expression

Mammalian

Mutation

codon-optimized ATG101-Y45D

Promoter

CMV

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-eDHFR(69K6)-iRFP713

Plasmid

#178855

Purpose

Mammalian expression of eDHFR(69K6)-iRFP713

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

eDHFR(69K6)-iRFP713

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCasper-pNos-MCP-mCherry

Plasmid

#180275

Purpose

to epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Article

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

MCP-mCherry

Tags

mCherry

Expression

Insect

Promoter

nanos

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJCC_164 SpCas9 R1333A/R1335A

Plasmid

#179528

Purpose

For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 R1333A/R1335A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginines 1333 and 1335 to alanine

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG1.1-Armc12 delta N/FLAG

Plasmid

#180495

Purpose

Expression vector of N-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

Insert

armadillo repeat containing 12 (Armc12 Mouse)

Tags

FLAG tag

Expression

Mammalian

Mutation

N-terminus of Armc12 is deleted

Promoter

CAG promoter

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG1.1-Armc12 delta C/FLAG

Plasmid

#180497

Purpose

Expression vector of C-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

Insert

armadillo repeat containing 12 (Armc12 Mouse)

Tags

FLAG tag

Expression

Mammalian

Mutation

C-terminus of Armc12 is deleted

Promoter

CAG promoter

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-hSPATA33-8xHIS-1D4

Plasmid

#179133

Purpose

Expression vector of human spermatogenesis associated 33 (SPATA33) tagged with 8XHIS and 1D4 at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

spermatogenesis associated 33 (SPATA33 Human)

Tags

1D4 and 8XHIS

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-WIPI2dR108E-cs-TEV-STREP

Plasmid

#171835

Purpose

Mammalian expression of human WIPI2d R108E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep

Expression

Mammalian

Mutation

R108E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-WIPI2dR125E-cs-TEV-STREP

Plasmid

#171836

Purpose

Mammalian expression of human WIPI2d R125E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep

Expression

Mammalian

Mutation

R125E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge