We narrowed to 5,538 results for: pcas

1. pspCas9-Klenow-ST2-com-vector

   Plasmid

   #176235

   Purpose

   Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG PSAM4 GlyR IRES EGFP

   Plasmid

   #119739

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   CAG

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV pCAG-FLEX-mScarlet-WPRE

   Plasmid

   #99280

   Purpose

   Can be used to generate AAV virus that will express mScarlet in the presence of Cre

   Depositor

   Rylan Larsen

   Insert

   mScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-MCP-dNS-SRRM1-V5-mCherry

   Plasmid

   #235095

   Purpose

   SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The SRRM1 entry clone from DNASU was modified usi…

   Promoter

   CAG

   Available since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG001 His6-TwinStrep-SUMO-AapCas12b

   Plasmid

   #153162

   Purpose

   Bacterial expression plasmid for AapCas12b

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

   Insert

   AapCas12b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

   Plasmid

   #181745

   Purpose

   dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   human/zebrafish codon optimized SpCas9
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-SpCas9(D10A)-T2A-EGFP

   Plasmid

   #221230

   Purpose

   Express nCas9 nickase (D10) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(D10A)-T2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

   Plasmid

   #167295

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAK (BAK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS SARS-CoV-2 XBB Spike

    Plasmid

    #195287

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 XBB Spike

    Depositor

    Marceline Côté

    Insert

    SARS-CoV-2 XBB Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19I, LPPA24-27…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    Feb. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCAG PSAM4 5HT3 LC IRES GFP

    Plasmid

    #123066

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G Q139L Y217F

    Promoter

    CAG

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

    Plasmid

    #190111

    Purpose

    AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    AAV

    Tags

    bpNLS

    Expression

    Mutation

    H840A

    Promoter

    EF1a

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-TWIN-STREP-FLAG-NPRL2

    Plasmid

    #164985

    Purpose

    NPRL2 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    NPRL2 (NPRL2 Human)
    

    Use

    Tags

    2xSTREP FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

    Plasmid

    #167296

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAX (BAX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-PspCas13b-non-targeting (pJK5)

    Plasmid

    #229052

    Purpose

    Cloning backbone for PspCas13b crRNA (U6 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. phU1-PspCas13b-non-targeting (pEZ36)

    Plasmid

    #229053

    Purpose

    Cloning backbone for PspCas13b crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0043-PspCas13b-gRNA non target

    Plasmid

    #223698

    Purpose

    gRNA control for dPspCas13b-FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    gRNA target sequence for luciferase control
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS SARS-CoV-2 Delta Spike

    Plasmid

    #185593

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 Delta Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 Delta Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19R, Δ156-157,…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pEN716 - pCAGGS-eGFP-3Xnls-bpA

    Plasmid

    #156435

    Purpose

    For transient expression of nuclear eGFP in mammalian cells

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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