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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 10,938 results for: NSI

Showing: 2021 - 2040 of 10938 results

  1. C101m

    Plasmid
    #121005
    Purpose
    MoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).
    Depositor
    Richard Murray
    Insert
    sfCFP with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121005-plasmid-map-sequence-id-236158

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  2. C20m

    Plasmid
    #120961
    Purpose
    MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)
    Depositor
    Richard Murray
    Insert
    sfYFP-ssrA (has BbsI site)
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120961-plasmid-map-sequence-id-235979

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  3. C22m

    Plasmid
    #120963
    Purpose
    MoClo golden gate assembly CD part for mSpinach (fluorescent RNA aptamer for DFHBI, suitable for in vivo use; see DOI: 10.1021/sb400089c). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mSpinach
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120963-plasmid-map-sequence-id-236357

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  4. P37m

    Plasmid
    #120947
    Purpose
    MoClo golden gate assembly AB part for pRpa* (improved pRpa from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pRpa* from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120947-plasmid-map-sequence-id-236378

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  5. P38m

    Plasmid
    #120948
    Purpose
    MoClo golden gate assembly AB part for pTra* (improved pTra from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pTra* from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120948-plasmid-map-sequence-id-236376

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  6. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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  8. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

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  9. T12m

    Plasmid
    #121031
    Purpose
    MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120029600 (strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121031-plasmid-map-sequence-id-249041

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  10. pJBL7346

    Plasmid
    #217370
    Purpose
    sfGFP reporter for synthetic transcriptional variant of Pca rhtB ZTP riboswitch
    Depositor
    Julius Lucks
    Article
    Bushhouse et al bioRxiv 2024.03.29.587317
    Insert
    Pca rhtB synthetic transcriptional riboswitch sfGFP reporter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    217370-plasmid-map-sequence-id-431670

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  12. T2m

    Plasmid
    #121029
    Purpose
    MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    ECK120033736 (164.6x Voigt terminator), short attachment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121029-plasmid-map-sequence-id-238076

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  13. T7m

    Plasmid
    #121030
    Purpose
    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    S. pyogenes tracrRNA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121030-plasmid-map-sequence-id-236182

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  14. T13m

    Plasmid
    #121032
    Purpose
    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120033737 (second strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121032-plasmid-map-sequence-id-236342

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  15. C100m

    Plasmid
    #121004
    Purpose
    MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121004-plasmid-map-sequence-id-236143

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  16. C102m

    Plasmid
    #121006
    Purpose
    MoClo golden gate assembly CD part treRT-LAA (synthetic Lac-family repressor chimera with LAA degradation tag for ssrA; see DOI: 10.1021/sb500262f).
    Depositor
    Richard Murray
    Insert
    treRT with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121006-plasmid-map-sequence-id-236159

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  17. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

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  18. P12m

    Plasmid
    #120938
    Purpose
    MoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pLasTetO
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120938-plasmid-map-sequence-id-256615

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  19. P15m

    Plasmid
    #120939
    Purpose
    MoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinTetO
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120939-plasmid-map-sequence-id-238035

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  20. P16m

    Plasmid
    #120940
    Purpose
    MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinLacO m
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120940-plasmid-map-sequence-id-238036

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Showing: 2021 - 2040 of 10938 results