Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 2,076 results for: Pam

Showing: 2021 - 2040 of 2076 results

  2. CFP-PCRD-FRB

    Plasmid
    #87449
    Purpose
    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels
    Depositor
    Xiaodong Liu
    Article
    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1
    Insert
    PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
    Use
    Tags
    6xHis-T7, CFP, and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87449-plasmid-map-sequence-id-174912

    Enlarge

  3. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid
    #222876
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FRB chain: V84R in GPA
    Promoter
    MSCV LTR
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222876-plasmid-map-sequence-id-441621

    Enlarge

  4. DRD3-DuET

    Plasmid
    #213229
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD3 (DRD3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213229-plasmid-map-sequence-id-430895

    Enlarge

  5. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

    Plasmid
    #185299
    Purpose
    Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    pvLEA22mer_shuffle_3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185299-plasmid-map-sequence-id-363926

    Enlarge

  6. DHN_VrDHN1a_mCh-SspB (pBS1074)

    Plasmid
    #185298
    Purpose
    Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    PvLEA4_repeats_k3_26
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185298-plasmid-map-sequence-id-363925

    Enlarge

  7. ApoE3_D125I_47-300_mCh-SspB (pBS1071)

    Plasmid
    #185294
    Purpose
    For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    ApoE3_D125I_47-300
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D125I
    Promoter
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185294-plasmid-map-sequence-id-363903

    Enlarge

  11. pvLEA22mer_mutant_3_mCh-SspB (pBS1080)

    Plasmid
    #185303
    Purpose
    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    pvLEA22mer_mutant_3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185303-plasmid-map-sequence-id-363934

    Enlarge

  12. PvLEA4_repeats_k5_1_mCh-SspB (pBS1079)

    Plasmid
    #185302
    Purpose
    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    PvLEA4_repeats_k5_1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185302-plasmid-map-sequence-id-363930

    Enlarge

  13. pvLEA22mer_mutant_10_mCh-SspB (pBS1078)

    Plasmid
    #185301
    Purpose
    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    pvLEA22mer_mutant_10
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185301-plasmid-map-sequence-id-363929

    Enlarge

  14. CAHS2_PARRC_98-130_mCh-SspB (pBS1077)

    Plasmid
    #185300
    Purpose
    For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    CAHS2_PARRC_98-130
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185300-plasmid-map-sequence-id-363927

    Enlarge

  15. DSUP_RAMVA_1-208_mCh-SspB (pBS1073)

    Plasmid
    #185296
    Purpose
    For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    DSUP_RAMVA_1-208
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185296-plasmid-map-sequence-id-363910

    Enlarge

  16. DHR93_Nccapped_mCh-SspB (pBS1072)

    Plasmid
    #185295
    Purpose
    For the mammalian expression of the synthetic protein DHR93_Nccapped attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    DHR93_Nccapped
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185295-plasmid-map-sequence-id-363905

    Enlarge

  20. LLPS2-CAHS2_PARRC::SspB (pBS1043)

    Plasmid
    #185290
    Purpose
    For the mammalian expression of the tardigrade protein CAHS2_PARRC attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    CAHS2_PARRC
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185290-plasmid-map-sequence-id-363897

    Enlarge

Showing: 2021 - 2040 of 2076 results