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Showing: 2021 - 2040 of 29407 results

  4. p-mCherry-N1-ChloC

    Plasmid
    #85461
    Purpose
    Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Peter Hegemann
    Article
    Wietek et al Science. 2014 Mar 27.
    Insert
    ChloC
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E90R, T159C
    Promoter
    CMV (+enhancer)
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85461-plasmid-map-sequence-id-170778

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  6. p-mCherry-N1-slowChloC

    Plasmid
    #85462
    Purpose
    Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Peter Hegemann
    Article
    Wietek et al Science. 2014 Mar 27.
    Insert
    slowChloC
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E90R, T159C, D156N
    Promoter
    CMV (+enhancer)
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85462-plasmid-map-sequence-id-170775

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  10. mCherry-CRY2-iSH2

    Plasmid
    #66839
    Purpose
    Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.
    Depositor
    Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    iSH2
    Use
    Tags
    mCherry-CRY2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    66839-plasmid-map-sequence-id-116784

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  13. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  18. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

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Showing: 2021 - 2040 of 29407 results