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ABE8.20-d

Plasmid

#136301

Purpose

expresses ABE8.20-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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PX459v3

Plasmid

#178799

Purpose

SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pC020 - LseCas13a from Listeria seeligeri

Plasmid

#91910

Purpose

Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LseCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Go1-CASE

Plasmid

#168123

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)

Use

Luciferase

Tags

cpVenus on GNG9

Expression

Mammalian

Mutation

NLuc is inserted at L91/G92 within GNAO1

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSBFP2-C1

Plasmid

#22880

Depositor

Dorus Gadella

Article

Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

Insert

SBFP2

Expression

Mammalian

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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AAV-HAPLN1

Plasmid

#200777

Purpose

Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

Depositor

Alexander Dityatev

Article

Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

Has Service

AAV8

Insert

Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)

Use

AAV

Tags

cfGFP

Promoter

Synapsin

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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LifeAct-LSSmGFP

Plasmid

#186534

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-E389-deltaCT

Plasmid

#8993

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

T389E, stop codon at amino acid 402

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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p4053 HA-E6AP isoform I

Plasmid

#8657

Depositor

Peter Howley

Article

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform I (UBE3A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NE

Plasmid

#184608

Purpose

For expression of a fluorescent sensor for norepinephrine in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NE

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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