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pACYCDuet_F31YL54IDHFR_WTTS

Plasmid

#91231

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31YL54I

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_G121VDHFR_R166QTS

Plasmid

#91232

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

G121V and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_L54IDHFR_R166QTS

Plasmid

#91234

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

L54I and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_L54IDHFR_WTTS

Plasmid

#91235

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

L54I

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_M42FDHFR_R166QTS

Plasmid

#91236

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42F and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pPLtetO-PdhR

Plasmid

#89655

Purpose

Plasmid for titrating synthesis of PdhR with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

Pyruvate dehydrogenase complex repressor

Use

Synthetic Biology

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pPLtetO-FtsB

Plasmid

#107559

Purpose

Plasmid for titrating synthesis of FtsB with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

ftsB

Use

Synthetic Biology

Promoter

pLtetO

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 S137D (#628)

Plasmid

#99944

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

S137D

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_N2A

Plasmid

#98677

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

N1775A, N1782A

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_del1961-1970

Plasmid

#98680

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with del1961-1970

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

del1961-1970

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_S1966C

Plasmid

#98681

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_N2A_S1966C

Plasmid

#98682

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

N1775A, N1782A, S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 S103W (#623)

Plasmid

#99941

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

S103W

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 S245X

Plasmid

#92372

Purpose

Inducible expression of M1S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 N184X

Plasmid

#92371

Purpose

Inducible expression of M1 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, N184X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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isoform PKC Epsilon (JCE592)

Plasmid

#89776

Purpose

yeast expression of mouse PKC Epsilon with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC Epsilon (Prkce Mouse)

Expression

Mammalian

Promoter

yeast PKC1 promoter (-600 to -1)

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-MAGE-112-429

Plasmid

#89128

Purpose

mammalian expression of human MAGE-A11 amino acid residues 112-429 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 112-429

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
const #7-RFP

Plasmid

#81081

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23109

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #6-RFP

Plasmid

#81080

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23117

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET24_BD3179

Plasmid

#73142

Purpose

Expresses in E.coli B. bacteriovorus Bd3179

Depositor

Sandra Gabelli

Article

de la Peña et al PLoS One. 2015 Nov 2;10(11):e0141716. do

Insert

Bd3179

Expression

Bacterial

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pPD95.75 RSF-1

Plasmid

#66863

Purpose

Generate transgenic C..elegans expressing GFP-fused RSF-1

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

RSF-1 (rsf-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pSG5 N-RFP SADBs

Plasmid

#67153

Purpose

pSG5 containing N-terminally RFP tagged mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

RFP

Expression

Mammalian

Promoter

SV40

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pSG5 N-HA SADBs

Plasmid

#67149

Purpose

pSG5 containing N terminally HA tagged mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

SV40, T7

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS_KDR

Plasmid

#65091

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed sequence to render plasmid resistant to s…

Promoter

CMV, 2x Tet

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestMYC_RBPMS_KDR

Plasmid

#65667

Purpose

MYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

MYC

Expression

Mammalian

Mutation

introduced HindIII site to exchange FLAG-HA tag w…

Promoter

CMV, 2x Tet

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_KDR_K100E

Plasmid

#65093

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed lysine 100 to glutamic acid (KDR backgrou…

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_KDR_F65A

Plasmid

#65092

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

change phenylalanine 65 to alanine (KDR backbone)

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_K36E_R38E

Plasmid

#65095

Purpose

Destination vector with RBPMS with K36E/R38E mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

change lysine 36 to glutamic acid and arginine 38…

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_KDR_K100A

Plasmid

#65663

Purpose

FLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed lysine 100 to alanine (KDR background)

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_K36E_R38A_E39A

Plasmid

#65658

Purpose

FLAG-HA expression vector with RBPMS carrying mutation K36E, R38A, E39A

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed lysine 36 to glutamic acid, arginine 38 t…

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge