We narrowed to 22,137 results for: his
-
Plasmid#233879PurposeExpresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213DepositorAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only
-
pXD80ENSF14-Pct3-ElVadh(cHis6)-Accs(ElDSM2243)
Plasmid#233871PurposeExpresses His-tagged Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)
Plasmid#233870PurposeExpresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)
Plasmid#233868PurposeExpresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(–)-Eldh2
UseTagsHisExpressionBacterialMutationPromoterAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)
Plasmid#233869PurposeExpresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-SARS-CoV-2-S-tail-Lys1269Ala-His1271Ala
Plasmid#218459PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Lys1269Ala and His1271Ala substitutions) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying Lys1269 and His1271 substitutions and an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationLys1269Ala and His1271Ala substitutionsPromoterAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A)
Plasmid#223751PurposeExpression of recombinant protein for purificationDepositorInsertWIPI2d (WIPI2 Human)
UseTags6xHis-TEV-mCherryExpressionBacterialMutationK87A/K88APromoterAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)
Plasmid#225328PurposeExpresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertHcdh partial gene cluster
UseTagsHis8ExpressionBacterialMutationPromoterAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA8-DadhA(cHis8)BC-DadRS-noAccs(ElA2)
Plasmid#225326PurposeExpresses Eggerthella lenta A2 Dadh in Eggerthella lenta DSM 2243DepositorInsertDadh
UseTagsHis8ExpressionBacterialMutationPromoterAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF7-DadhA(cHis8)BC-DadRS-Accs(ElA2)
Plasmid#225302PurposeExpresses Eggerthella lenta A2 Dadh in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh
UseTagsHis8ExpressionBacterialMutationPromoterAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF8-HcdhA(cHis8)BC-HcdR-Accs(ElA2)
Plasmid#225303PurposeExpresses Eggerthella lenta A2 Hcdh in Gordonibacter urolithinfaciens DSM 27213DepositorInsertHcdh
UseTagsHis8ExpressionBacterialMutationPromoterAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)
Plasmid#233876PurposeExpresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh
UseTagsHis8ExpressionBacterialMutationPromoterAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP-TEV-Rat BCKDK-His
Plasmid#232119PurposeFor bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.DepositorInsertBCKDK (Bckdk Rat)
UseTags6 x His, Maltose-Binding Protein (MBP), and Tev S…ExpressionBacterialMutationPromoterT7 PromoterAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS3-rab-3P::AI::lite-1G::SL2::his-24::tagBFP
Plasmid#232610PurposePan-neuronal expression of 'LITE-1' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTRDepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP
Plasmid#232608PurposePan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTRDepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS3-rab-3P::AI::prdx-2G::SL2::his-24::tagRFP
Plasmid#232609PurposePan-neuronal expression of 'PRDX-2' using a genomic fragment and fluorophore 'tagRFP', regulated by rab-3 promoter and unc-54 3' UTRDepositorAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual His6-TEV-CUL2, Rbx1
Plasmid#231000PurposeDual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cellsDepositorAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+Strep-Opa1 (SB254)
Plasmid#227603PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastorisDepositorInsertsUseTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)
Plasmid#227608PurposeInducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationaa 2-83 deletedPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)
Plasmid#227609PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-544, 651-757 only [TM deleted]PromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2-Strep (SB255)
Plasmid#227604PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-HIS3MX6
Plasmid#228023PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-HISMX6
Plasmid#228027PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with HISMX6 markerDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HIS3p-TID-FRB-NLS-HA3
Plasmid#228005PurposeExpresses TurboID-FRB-NLS fusion from HIS3 promoter; yeast BioIDDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterHIS3Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HIS3p- TID-FKBP-NLS-HA3
Plasmid#228007PurposeExpresses TurboID-FKBP-NLS fusion from HIS3 promoter; yeast BioIDDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterHIS3Available SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46[R247A,R347A]-His (SB257)
Plasmid#227606PurposeInducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastorisDepositorInsertSLC25A46 (SLC25A46 Human)
UseTags10xHisExpressionYeastMutationR247A,R347APromoterAOX1Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L (381-975)_pcDNA3.1(+)
Plasmid#187725PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 1-380PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L (492-975)_pcDNA3.1(+)
Plasmid#187726PurposeExpress his-tagged NEDD4L W3-W4-HECT in mammalian cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 1-491PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-FIVAR-ELP-HIS-ybbR
Plasmid#169136PurposeE. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertFIVAR (found in various architectures)
UseTags3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…ExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CTLA-4-GCN4-His6
Plasmid#223582PurposeProtein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cellsDepositorInsertCTLA4 (CTLA4 Human)
UseTagsGCN4, His6 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CD80 (I92R)-His10
Plasmid#223580PurposeProtein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cellsDepositorInsertCD80 (CD80 Human)
UseTagsHis10 and Twin-Strep, SNAPExpressionMammalianMutationI92RPromoterAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9-FLAG
Plasmid#217328PurposePurification of rat DPP9 fusion with C-terminal FLAG tag from insect cellsDepositorInsertDPP9
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9(S729A)
Plasmid#217327PurposePurification of catalytically-dead rat DPP9 from insect cellsDepositorInsertDPP9
UseTagsHis-TEVExpressionInsectMutationS729APromoterpolyhedrinAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1-FLAG
Plasmid#217323PurposePurification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala
Plasmid#218463PurposeMammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)DepositorInsertSARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )
UseTagsExpressionMammalianMutationLys1269Ala and His1271Ala substitutionsPromoterAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC120-MBP-DDX3X(D354V)-monoGFP-His
Plasmid#219986PurposeDDX3X(D354V) protein purificationDepositorInsertDDX3X(D354V) (DDX3X Human)
UseTagsMBP and monoGFP and 6xHisExpressionInsectMutationD354VPromoterAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC120-MBP-DDX3X(G325E)-monoGFP-His
Plasmid#219987PurposeDDX3X(G325E) protein purificationDepositorInsertDDX3X(G325E) (DDX3X Human)
UseTagsMBP and monoGFP and 6xHisExpressionInsectMutationG325EPromoterAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN002P-M-FLAG-His
Plasmid#218026PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN002P-M
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable SinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN002X-N-FLAG-His
Plasmid#218033PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN002X-N
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable SinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN002W-V-FLAG-His
Plasmid#218024PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN002W-V
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable SinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only