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p3E-rab38a

Plasmid

#109699

Purpose

Multisite gateway vector for 3' tagging with rab38a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab38a (rab38a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab32b

Plasmid

#109690

Purpose

Multisite gateway vector for 3' tagging with rab32b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab32b (rab32b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab39a

Plasmid

#109632

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab39a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab39a

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-EHD2a

Plasmid

#109568

Purpose

Multisite gateway vector for 3' tagging with EHD2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EHD2a

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-CaaX(tK)

Plasmid

#109540

Purpose

Multisite gateway vector for 3' tagging with the CaaX domain from Kras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX domain from Kras

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p5343_UC57

Plasmid

#126645

Purpose

environmental plasmid p5343 cloned into pUC57

Depositor

Aindrila Mukhopadhyay

Article

Kothari et al mSystems. 2019 Dec 17;4(6). pii: 4/6/e00

Insert

Environmental plasmid p5343

Use

Synthetic Biology

Promoter

NA

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSADuet148

Plasmid

#121910

Purpose

Charge-introduced NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBDuet259

Plasmid

#121911

Purpose

NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

NpuDnaBΔ283ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 283-residue endonuclease domain

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD113

Plasmid

#121912

Purpose

Charge-Introduced NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBC39 intein

Tags

GB1-His6

Expression

Bacterial

Mutation

S110K, I111K

Promoter

Para

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBDuet116

Plasmid

#121915

Purpose

NpuDnaBΔ290ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBΔ290ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD168

Plasmid

#121916

Purpose

Oth-NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBC39 intein

Tags

GB1-His6

Expression

Bacterial

Mutation

I111K, E116K

Promoter

Para

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLR5-U6-sgRNA-EF

Plasmid

#82635

Purpose

piggyBac vector for expressing modified sgRNA in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Type

Empty backbone

Use

CRISPR; Piggybac vector

Expression

Mammalian

Promoter

U6

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP38-1A(sfGFP)

Plasmid

#126984

Purpose

Level 1 vector with alternative selection marker (chloramphenicol). Origin pUC (high copy number). sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pcue_P

Plasmid

#127009

Purpose

Basic Part P- Promoter (non-constitutive); Pcue; metal sensitive; controlled by CueR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pcue_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A.pcDNA3-k

Plasmid

#126627

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-L2U2H09_T

Plasmid

#127042

Purpose

Basic Part T- Terminator; L2U2H09; synthetic terminator

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L2U2H09_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-L1U1H08_CT

Plasmid

#127050

Purpose

Basic Part CT- C-terminus + Terminator; L1U1H08. C-termini STOP + terminator (synthetic)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L1U1H08_CT

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-L1U1H09_CT

Plasmid

#127049

Purpose

Basic Part CT- C-terminus + Terminator; L1U1H09. C-termini STOP + terminator (synthetic)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L1U1H09_CT

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-L2U2H09_CT

Plasmid

#127048

Purpose

Basic Part CT- C-terminus + Terminator; L2U2H09. C-termini STOP + terminator (synthetic)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L2U2H09_CT

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-L3S2P21_CT

Plasmid

#127046

Purpose

Basic Part CT- C-terminus + Terminator; L3S2P21. C-termini STOP + terminator (synthetic)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S2P21_CT

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-B0015_CT

Plasmid

#127045

Purpose

Basic Part CT- C-terminus + Terminator; B0015. C-termini STOP + Terminator.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0015_CT

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-L1U1H08_T

Plasmid

#127044

Purpose

Basic Part T- Terminator; L1U1H08; synthetic terminator

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L1U1H08_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-L1U1H09_T

Plasmid

#127043

Purpose

Basic Part T- Terminator; L1U1H09; synthetic terminator

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L1U1H09_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-L3S1P11_T

Plasmid

#127041

Purpose

Basic Part T- Terminator; L3S1P11; synthetic terminator

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P11_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-L3S1P32_T

Plasmid

#127040

Purpose

Basic Part T- Terminator; L3S1P32; synthetic terminator

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P32_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-L3S2P21_T

Plasmid

#127039

Purpose

Basic Part T- Terminator; L3S2P21; synthetic terminator

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S2P21_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-B0015_T

Plasmid

#127038

Purpose

Basic Part T- Terminator; B0015. Terminator.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0015_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-Histag_N

Plasmid

#127033

Purpose

Basic Part N- N-terminus; polyhistidine tag

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part His6_N

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-cI-ts_O

Plasmid

#127030

Purpose

Basic Part O- ORF; cI; transcription factor from lambda phage; thermosensitive.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part cI-ts_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

cI thermosensitive mutation

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-cI_O

Plasmid

#127029

Purpose

Basic Part O- ORF; cI; transcription factor from lambda phage.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part cI_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-lacZ_O

Plasmid

#127027

Purpose

Basic Part O- ORF; lacZ alpha-peptide; blue/white screening.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part lacZ_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-lacI_O

Plasmid

#127024

Purpose

Basic Part O- ORF; lacI; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part LacI_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-arsR_O

Plasmid

#127022

Purpose

Basic Part O- ORF; arsR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part arsR_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-araC_O

Plasmid

#127020

Purpose

Basic Part O- ORF; araC; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part araC_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-amilCP_O

Plasmid

#127019

Purpose

Basic Part O- ORF; amilCP; blue chromoprotein

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part amilCP_O

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-sfGFP_O

Plasmid

#127018

Purpose

Basic Part O- ORF; sfGFP; fluorescent protein

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part sfGFP_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-RBS-pET_R

Plasmid

#127013

Purpose

Basic Part R- RBS; pET vector Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part RBS-pET_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-B0034_R

Plasmid

#127012

Purpose

Basic Part R- RBS; B0034m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0034m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-B0033_R

Plasmid

#127011

Purpose

Basic Part R- RBS; B0033m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0033m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-B0032_R

Plasmid

#127010

Purpose

Basic Part R- RBS; B0032m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0032m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-Pmer_P

Plasmid

#127008

Purpose

Basic Part P- Promoter (non-constitutive); Pmer; metal sensitive; controlled by merR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pmer_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-Pars_P

Plasmid

#127007

Purpose

Basic Part P- Promoter (non-constitutive); Pars; metal sensitive; controlled by arsR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pars_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-Pci_P

Plasmid

#127006

Purpose

Basic Part P- Promoter (non-constitutive); Pci; controlled by cI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pci_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23119s_P

Plasmid

#127001

Purpose

Basic Part P- Promoter (constitutive); J23119 (1bp short on 3').

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23119s_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23106_P

Plasmid

#126999

Purpose

Basic Part P- Promoter (constitutive); J23106.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23106_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23103_P

Plasmid

#126998

Purpose

Basic Part P- Promoter (constitutive); J23103.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23103_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pClodANL-FGF2NBAA

Plasmid

#107873

Purpose

Bacterial expression of FGF2 minimum export mutant-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-minimal export

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

C77A, C95A, K128Q, R129Q, K134Q

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS406 ATG9-GFP T119D

Plasmid

#114668

Purpose

Expresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Promoter

ATG9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE.zeo/hKGA.S286A

Plasmid

#110357

Purpose

Mammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tag

Depositor

Sandra Martha Gomes Dias

Insert

KGA glutaminase (GLS Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Mutation

S286A

Promoter

gag

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PP245

Plasmid

#78965

Purpose

Promoter: GAP, minimal Promoter: AOX1. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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