We narrowed to 3,045 results for: ER;

1. pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

   Plasmid

   #162386

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8s
   

   Tags

   6xHis

   Expression

   Insect

   Mutation

   S26M F286Y Q315H

   Promoter

   hsp70

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

   Plasmid

   #170995

   Purpose

   HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ef1-alpha

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CMV-jGCaMP7c variant 1513

   Plasmid

   #105320

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7c variant 1513
   

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET iGluSnFR3 v82

   Plasmid

   #175187

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   iGluSnFR3 v82
   

   Use

   Synthetic Biology

   Tags

   His tag, T7 leader, Xpress epitope

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-xF2X-P2A-Puro

   Plasmid

   #110873

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS-2X(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

   Plasmid

   #162388

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-TFIIE

   Plasmid

   #171083

   Purpose

   Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   TF2E1 (GTF2E1 Human)
   TF2E2 (GTF2E2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.hSynap-FLEX.SF-iGluSnFR.A184S

   Plasmid

   #106180

   Purpose

   Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV5

   Insert

   SF-iGluSnFR.A184S
   

   Use

   AAV

   Mutation

   GltI: A184S

   Promoter

   hSynapsin

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFPC1-hVAP-B KD/MD

   Plasmid

   #104450

   Purpose

   Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein B (VAPB Human)
   

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   changed K87 to D and M89 to D

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

    Plasmid

    #236231

    Purpose

    AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2-elavl3-Voltron-ST

    Plasmid

    #119038

    Purpose

    Pan neuronal expression of soma-localized Voltron in zebrafish

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron-ST
    

    Use

    Zebrafish expression

    Promoter

    elavl3

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid

    #222497

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hsyn-flex-Voltron

    Plasmid

    #119035

    Purpose

    Cre-dependant expression of Voltron in neurons

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hsyn

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162383

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162387

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-STAT-CA

    Plasmid

    #195576

    Purpose

    Expresses STAT3 constitutively active mutant

    Depositor

    Kevin Park

    Article

    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

    Insert

    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    changed Alanine 662 to Cysteine and Asparagine 66…

    Promoter

    CMV enhancer and promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc-mCh-Tub

    Plasmid

    #24288

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Alpha-Tubulin (alphaTub84B Fly)
    

    Tags

    mCherry

    Expression

    Insect

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-hsyn-flex-Voltron-ST

    Plasmid

    #119036

    Purpose

    Cre-dependant expression of soma-localized Voltron in neurons

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Has Service

    AAV1

    Insert

    Voltron-ST
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hsyn

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV hSyn INTRON mScarlet-CAAX2 WPRE

    Plasmid

    #236232

    Purpose

    AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mScarletI fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mScarletI

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3-AUP1-FLAG-DATDCUE

    Plasmid

    #185340

    Purpose

    Assessing domain requirement sof AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Tags

    FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    deletion of aa 90-337 corresponding to the acyl t…

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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