We narrowed to 10,458 results for: T7

1. pAIP4_3TOW

   Plasmid

   #234201

   Purpose

   Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3TOW (MVB12B Human)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pAIP4_1KQX

   Plasmid

   #234181

   Purpose

   Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1KQX (rbp2a Zebrafish)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pAIP4_1AKO

   Plasmid

   #234162

   Purpose

   Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1AKO (xthA Escherichia coli K-12)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pAIP4_4NFB

   Plasmid

   #234161

   Purpose

   Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   4NFB (PILRA Human)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268

   Plasmid

   #227089

   Purpose

   Bacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)

   Depositor

   Chris Hill

   Insert

   tom1p-HECT (TOM1 Budding Yeast)
   

   Use

   Tags

   HIS-MBP-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

   Plasmid

   #227086

   Purpose

   Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Use

   Tags

   GST-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   R1282H

   Promoter

   T7

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pet28a+_Yjjg

   Plasmid

   #220228

   Purpose

   Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar

   Depositor

   Bernd Nidetzky

   Insert

   Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pet15b _yein

   Plasmid

   #220226

   Purpose

   Bacterial expression of YeiN in E. coli BL21_DE3 (pLys) or similar

   Depositor

   Bernd Nidetzky

   Insert

   psug (psuG E.coli)
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 8VL7

   Plasmid

   #220883

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pET28-Flag-RBM24

    Plasmid

    #208621

    Purpose

    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7CFE1_EVA71_VP12A

    Plasmid

    #212977

    Purpose

    Encodes EV-A71 4643 VP12A optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Enterovirus A71 4643 VP12A
    

    Use

    Mammalian cell free protein expression

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQE80l B-CC-B

    Plasmid

    #196479

    Purpose

    Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC

    Depositor

    Fei Sun

    Insert

    B-CC-B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Talin1-FERM

    Plasmid

    #110563

    Purpose

    bacterial expression of Talin 1

    Depositor

    Tina Izard

    Insert

    Talin 1 (Tln1 Mouse)
    

    Use

    Tags

    (His)8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TBXTA-c022

    Plasmid

    #139759

    Purpose

    Protein expression/crystallography

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, G177D (TBXT Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids E41-A214

    Promoter

    T7

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmSod1-Q42L;Q123A-N’His

    Plasmid

    #117710

    Purpose

    mutation on stability/solubility of SOD1

    Depositor

    Elizabeth Fisher

    Insert

    Sod1 (Sod1 Mouse)
    

    Use

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Q42L; Q123A

    Promoter

    T7

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACYCduet-mxLOX2-EH

    Plasmid

    #104979

    Purpose

    Biosynthesis of oxylipins by microbial enzymes

    Depositor

    Deokkun Oh

    Inserts

    MXAN_1744 (MXAN_1744 Myxococcus xanthus)
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 5K29 (PP-BRD3)

    Plasmid

    #101883

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. 5TCM (PP-BRD3)

    Plasmid

    #101888

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Mmadhc

    Plasmid

    #74662

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    Mmadhc (MMADHC Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pJK611

    Plasmid

    #72532

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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