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pTC363

Plasmid

#91211

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtU6 promoters)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC303

Plasmid

#91210

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTaCas9-H840A_6

Plasmid

#91173

Purpose

Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_H840A (nickase)

Use

CRISPR

Expression

Plant

Mutation

H840A

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTaCas9-D10A_6

Plasmid

#91171

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-dead_3

Plasmid

#91167

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_3

Plasmid

#91161

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1310

Plasmid

#91034

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_H840A nickase

Use

CRISPR

Mutation

H840A

Promoter

ZmUbi

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1410

Plasmid

#91035

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

ZmUbi

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0208

Plasmid

#91004

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

EC1.2

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pZS1[LacI-L]

Plasmid

#60745

Purpose

Contains PI driving expression dimeric LacI, the Lactose inducible repressor.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

LacI

Use

Synthetic Biology

Expression

Bacterial

Mutation

wt LacI without the tetramerization domain

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET30a-SARS-CoV2-ORF8

Plasmid

#165089

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Publication

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)

Expression

Bacterial

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET30a-SARS-CoV2-ORF3b (S23Q)

Plasmid

#165088

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Publication

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)

Expression

Bacterial

Mutation

Stop codon to glutamine at 23rd amino acid

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSC4

Plasmid

#91182

Purpose

T-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting 58kb region in medicago truncatula

Use

CRISPR

Expression

Plant

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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DBJS-p8.35

Plasmid

#246898

Purpose

Used for multi-site piggybac transposon insertion of luciferase reporter

Depositor

David Bartel

Insert

CNOT10::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac

Expression

Mammalian

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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DBJS-p8.38

Plasmid

#246899

Purpose

Used for multi-site piggybac transposon insertion of luciferase reporter

Depositor

David Bartel

Insert

DDIT4L::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac

Expression

Mammalian

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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DBJS-p8.32

Plasmid

#246897

Purpose

Used for multi-site piggybac transposon insertion of luciferase reporter

Depositor

David Bartel

Insert

RB1::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac

Expression

Mammalian

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pN1_HsKIF1C(349-1103)-mNG

Plasmid

#253327

Purpose

mammalian expression of the stalk+tail domains of human KIF1C (aa 349-1103) tagged with mNG.

Depositor

Kristen Verhey

Publication

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(349-1103) (KIF1C Human)

Tags

mNeonGreen

Expression

Mammalian

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-myr-rsEGFP2- LDLR

Plasmid

#213399

Purpose

double floxed, myristoylation site (myr) and LDLR, fused to reversibly switchable EGFP rsEGFP2

Depositor

Katrin Willig

Publication

Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

Insert

rsEGFP2

Use

AAV

Tags

C-terminal (Ct) cytoplasmic domains of low densit…

Expression

Mammalian

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRC043

Plasmid

#206799

Purpose

J23107.SoxS-GID1

Depositor

James Carothers

Publication

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

SoxS-GID1

Promoter

J23107

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRC025

Plasmid

#206796

Purpose

J23107.MCP-ABI

Depositor

James Carothers

Publication

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

MCP-ABI

Promoter

J23107

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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