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pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

Plasmid

#227009

Purpose

Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

Plasmid

#227010

Purpose

Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

Plasmid

#227011

Purpose

Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

Plasmid

#227012

Purpose

Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
E67K SUMO1

Plasmid

#221065

Purpose

Expression of E67K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

E67K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

E67K

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35K SUMO1

Plasmid

#221055

Purpose

Expression of H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35K

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35S SUMO1

Plasmid

#221056

Purpose

Expression of H35S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35S site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35S

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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E67R SUMO1

Plasmid

#221064

Purpose

Expression of E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

E67R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

E67R

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H43R SUMO1

Plasmid

#221057

Purpose

Expression of H43R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H43R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H43R

Promoter

T7

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H35R SUMO1

Plasmid

#221054

Purpose

Expression of H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35R

Promoter

T7

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge