We narrowed to 5,258 results for: pcDNA3.1+

1. pcDNA3.1-Gα13(EE)-YFP

   Plasmid

   #171374

   Purpose

   Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Ga13(EE)-YFP
   

   Use

   Tags

   Glu-Glu

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1(+)MBII-85-5'/3'ss mut

   Plasmid

   #67645

   Purpose

   MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII85 snoRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5' and 3' splice sites mutations

   Promoter

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Myc-gamma3 (in pCDNA3.1)

   Plasmid

   #118041

   Purpose

   GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG3 (Gabrg3 Rat)
   

   Use

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Myc-gamma1 (in pCDNA3.1)

   Plasmid

   #118038

   Purpose

   GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG1 (Gabrg1 Rat)
   

   Use

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Nluc(W12stop)

   Plasmid

   #180502

   Purpose

   Mammalian expression of Nluc(W12stop)

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   Nluc(W12stop)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   W12(UGG) to amber stop codon(UAG)

   Promoter

   CMV

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-FluoSTEP-EKAR

   Plasmid

   #181858

   Purpose

   Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-EKAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-nAChr-beta3

   Plasmid

   #48811

   Purpose

   This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

   Depositor

   Jerry Stitzel, Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   *

   Promoter

   CMV

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. a4 ecto pcDNA3.1 hygro(-)

   Plasmid

   #113406

   Purpose

   expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

   Depositor

   Timothy Springer

   Article

   Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

   Insert

   human alpha 4 ectodomain
   

   Use

   Tags

   Strep II tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CCND1(T286A)

   Plasmid

   #172631

   Purpose

   Expresses untagged cyclin D1(T286A) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Thr286Ala

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. XE212 RGS9-pcDNA3.1

    Plasmid

    #16676

    Depositor

    Randall Moon

    Insert

    RGS9 (Rgs9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only