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pSANG10-3F-BG4

Plasmid

#55756

Purpose

Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

Depositor

Shankar Balasubramanian

Article

Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

Insert

BG4

Use

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQAT03

Plasmid

#223375

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-SpCas9-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT05

Plasmid

#223377

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpCas9-OsU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTRANS_210d

Plasmid

#91109

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_220

Plasmid

#91113

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_220d

Plasmid

#91114

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGreen_dualluc_ORF_sensor

Plasmid

#55207

Purpose

Dual-luciferase reporter for microRNA targeting in plants

Depositor

Michael Axtell

Article

Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

Inserts

Renilla luciferase
Firefly luciferase

Use

Luciferase; Plant transient expression

Tags

Expression

Mutation

Promoter

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Use

Tags

6xHis-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_210

Plasmid

#91108

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_201

Plasmid

#91105

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_260d

Plasmid

#91126

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

PvUbi2

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_150

Plasmid

#91205

Purpose

Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_100

Plasmid

#91198

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF124

Plasmid

#222372

Purpose

Recombinant human IgG1 antibody (3D5) that binds to 6xHis at the C-terminus of proteins

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTRANS_250d

Plasmid

#91124

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

PvUbi2

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_233

Plasmid

#91122

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_101

Plasmid

#91199

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_160

Plasmid

#91206

Purpose

Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_232

Plasmid

#91121

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_102

Plasmid

#91200

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge