We narrowed to 3,920 results for: pCMV mammalian

1. pCMV-myc-Rem2 RNAiR2 short

   Plasmid

   #51596

   Purpose

   expresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2 Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   silent mutations at shRNA 1 and 2 recognition sit…

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

   Plasmid

   #185911

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

   Plasmid

   #185910

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpCas9-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A

   Promoter

   CMV and T7

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

   Plasmid

   #185916

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

   Plasmid

   #185915

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

   Plasmid

   #185918

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-FLEx-TetOn3G-3xHA-FLEx

   Plasmid

   #133729

   Purpose

   Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   TetOn3G-3xHA (floxed & inverted)
   

   Use

   AAV

   Tags

   3xHA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-sport2-mGCN5 FU, FLAG Tagged

   Plasmid

   #23098

   Depositor

   Sharon Dent

   Article

   Martinez-Balbas et al EMBO J. 2000 Feb 15. 19(4):662-71.

   Insert

   mouse Gcn5 mRNA (Kat2a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   missing 50 bp upstream of ATG start site.

   Promoter

   Available since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-Flag-YAP-S94/F95A

    Plasmid

    #33057

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S94A, F95A

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-RA-HRas-IRES2-Lyn-iRFP682

    Plasmid

    #180584

    Purpose

    RA-Hras and Lyn-iRFP682

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    RA-Hras and Lyn-iRFP682
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMSSM97: pCMV-HA-NLS-SpuFz1-E498R

    Plasmid

    #205275

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498R

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    E498R

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMSSM105: pCMV-HA-NLS-SpuFz1-T513K

    Plasmid

    #205276

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513K

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    T513K

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSSM58: pCMV-HA-NLS-SpuFz1-D487K

    Plasmid

    #205274

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487K

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    D487K

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSSM59: pCMV-HA-NLS-SpuFz1-D300R

    Plasmid

    #205272

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300R

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    D300R

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV Lex VP16 (F442A) HA (P#1710)

    Plasmid

    #14595

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Use

    Tags

    HA and VP16 (F442A)

    Expression

    Mammalian

    Mutation

    The VP16 sequence contains an F442A mutation. The…

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pCMV Ub Lex VP16 (F442A) HA (P#1711)

    Plasmid

    #14596

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Use

    Tags

    HA, Ubiquitin, and VP16 (F442A)

    Expression

    Mammalian

    Mutation

    The VP16 sequence contains an F442A mutation. The…

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid

    #92390

    Purpose

    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid

    #185917

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge