We narrowed to 7,564 results for: ALP

1. pCA528 CAPN7 V18D,F98D (1-165)

   Plasmid

   #193034

   Purpose

   Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   V18D, F98D, Residues 1-165

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSuper Retro GFP DGK alfa human

   Plasmid

   #224578

   Purpose

   To stably downregulate the expression of human DGK alfa in human cells

   Depositor

   Isabel Mérida

   Article

   Torres-Ayuso et al Oncotarget. 2014 Oct 30;5(20):9710-26. d

   Insert

   sh RNAi Diacylglycerol kinase alfa human (DGKA Human)
   

   Use

   RNAi and Retroviral

   Promoter

   H1

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ADRA2B-DuET

   Plasmid

   #213178

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRA2B (ADRA2B Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-SopB(172-554)

   Plasmid

   #183664

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 172-554

   Promoter

   CMV

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-SopB(211-554)

   Plasmid

   #183665

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 211-554

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

   Plasmid

   #60394

   Purpose

   Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB1 (TUB1 Synthetic, Budding Yeast)
   

   Expression

   Yeast

   Mutation

   internal 6xHis (see supplement figure 4), amino a…

   Promoter

   GAL

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

7. HNF4A_P2-198

   Plasmid

   #31065

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

   Insert

   HNF4A P2 promoter (HNF4A Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   -198 to -1 of P2 HNF4A promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-7 a-syn A53T D115A mNeonGreen-3K-B11

   Plasmid

   #232017

   Purpose

   Bacterial expression of alpha synuclein A53T mutant, tagged with the final beta strand of mNeonGreen-3K

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   SNCA (SNCA Human)
   

   Tags

   mNeonGreen-3K beta11

   Expression

   Bacterial

   Mutation

   Ala53Thr Asp115Ala

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZL-Blast FAP S624A

   Plasmid

   #207402

   Purpose

   Expresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.

   Depositor

   Jing Yang

   Article

   Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

   Insert

   Fibroblast Activation Protein Alpha (FAP Human)
   

   Use

   Retroviral

   Mutation

   changed Serine 624 to Alanine. It's a protea…

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZL-Blast FAP A657S

    Plasmid

    #207403

    Purpose

    Expresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.

    Depositor

    Jing Yang

    Article

    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

    Insert

    Fibroblast Activation Protein Alpha (FAP Human)
    

    Use

    Retroviral

    Mutation

    changed Alanine 657 to Serine. It's an endop…

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CMV-MARCer-Blast

    Plasmid

    #160550

    Purpose

    labeling hypoxic cells

    Depositor

    Marc Vooijs

    Article

    Vermeer et al Dis Model Mech. 2020 Jul 30;13(7). pii:

    Insert

    MARCer (HIF1A Human)
    

    Use

    Lentiviral

    Tags

    eGFP-Cre

    Promoter

    CMV

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-2dV-Camui (wt)

    Plasmid

    #220366

    Purpose

    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CRISPRv2-sgIl1r1_#2-hygro

    Plasmid

    #231990

    Purpose

    Knockout mouse Il1r1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-DmrB-Casp8-2A-mCherry-puro

    Plasmid

    #231971

    Purpose

    Tet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Casp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGM238

    Plasmid

    #220178

    Purpose

    Expresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Mutation

    K78E (NACA) + K43B (BTF3)

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFPC1-hVAP-A KD/MD

    Plasmid

    #104449

    Purpose

    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed K94 to D and M96 to D

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0481

    Plasmid

    #142638

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIF1A (HIF1A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-HIF2α P405A Δ450-572-pBabe-Puro

    Plasmid

    #25958

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. TOPO-HIF1A

    Plasmid

    #226452

    Purpose

    For subcloning of human HIF1A promoter or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    HIF1A (HIF1A Human)
    

    Use

    Cloning vector

    Mutation

    No

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFPC1-hVAP-B KD/MD

    Plasmid

    #104450

    Purpose

    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    changed K87 to D and M89 to D

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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