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Addgene

We narrowed to 2,112 results for: AP1

Showing: 2061 - 2080 of 2112 results
  1. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Insert
    NES-ZapCV2
    Use
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. TRIPZ-bioGEF-SUMO1nc-PURO

    Plasmid
    #208042
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Insert
    KRas c-term (KRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. Anti-Shank2 [N23B/6R-2b]

    Plasmid
    #219475
    Purpose
    Mammalian Expression Plasmid of anti-Shank2 scaffold protein (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N23B/6.
    Depositor
    Insert
    Anti-Shank2 scaffold protein (Rattus norvegicus) recombinant mouse monoclonal antibody. (Shank2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 2061 - 2080 of 2112 results