We narrowed to 4,126 results for: Alk

1. pLC045 - pL0_CrGLK (CDS1ns)

   Plasmid

   #203904

   Purpose

   MoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   CrGLK (without a stop codon)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Silent mutation of BpiI or BsaI cut site for MoCl…

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUN1213 - pL0_p16OMT (pro + 5U)

   Plasmid

   #203890

   Purpose

   16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   16OMT promoter and 5'UTR
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Mutation of BpiI and/or BsaI cut sites for MoClo …

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUN1214 - pL0_pNMT (pro + 5U)

   Plasmid

   #203893

   Purpose

   NMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   NMT promoter and 5'UTR
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Mutation of BpiI and/or BsaI cut sites for MoClo …

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)GαsS-246-Nluc

   Plasmid

   #207157

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAS (GNAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position D(246)N

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)GαsL-260-NLuc

   Plasmid

   #207158

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAS (GNAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position D(260)N

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

   Plasmid

   #196878

   Purpose

   Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-N-gTBD
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

   Plasmid

   #196881

   Purpose

   Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-Cdk5rap2-CTD
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD

   Plasmid

   #148514

   Purpose

   Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD

   Plasmid

   #148515

   Purpose

   Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-675

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   DmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-MS2-HA-C1-HsGIGYF2_1-718_AI

    Plasmid

    #148960

    Purpose

    Mammalian Expression of HsGIGYF2_1-718

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2_1-718 (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7-EGFP-C1-HsGIGYF2_1-718_AI

    Plasmid

    #148948

    Purpose

    Mammalian Expression of HsGIGYF2_1-718

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2_1-718 (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7-EGFP-C1-HsGIGYF2_1-718-YFWFtoAAAA_AI

    Plasmid

    #148950

    Purpose

    Mammalian Expression of HsGIGYF2_1-718-YFWFtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2_1-718-YFWFtoAAAA (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-EGFP-C1-HsGIGYF2_280-310_AI

    Plasmid

    #148951

    Purpose

    Mammalian Expression of HsGIGYF2_280-310

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2_280-310 (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT7-EGFP-C1-HsGIGYF2-F300AF306A_AI

    Plasmid

    #148952

    Purpose

    Mammalian Expression of HsGIGYF2-F300AF306A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2-F300AF306A (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7-EGFP-C1-HsGIGYF2-W288A_AI

    Plasmid

    #148953

    Purpose

    Mammalian Expression of HsGIGYF2-W288A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2-W288A (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-EGFP-C1-HsGIGYF2-W288AF300AF306A_AI

    Plasmid

    #148954

    Purpose

    Mammalian Expression of HsGIGYF2-W288AF300AF306A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2-W288AF300AF306A (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7-EGFP-C1-HsGIGYF2-YYMLtoAAAA_AF

    Plasmid

    #148691

    Purpose

    Mammalian Expression of HsGIGYF2-YYMLtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF2-YYMLtoAAAA (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF

    Plasmid

    #148686

    Purpose

    Bacterial Expression of HsGIGYF2_35-105-E46AE47FN91A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF2_35-105-E46AE47FN91A (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)-C-6His-mRplp1

    Plasmid

    #173153

    Purpose

    mouse Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) expression plasmid in mammalian cells

    Depositor

    Brijesh Kumar Singh

    Article

    Tripathi et al bioRxiv 2021.11.16.468891

    Insert

    ORF clone Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) (Rplp1 Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX026

    Plasmid

    #167153

    Purpose

    MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnLuz-ocsT
    

    Use

    Luciferase

    Tags

    His6-tag

    Expression

    Plant

    Mutation

    Promoter

    p35s

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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