We narrowed to 5,822 results for: pCas

1. pC0043-PspCas13b-gRNA non target

   Plasmid

   #223698

   Purpose

   gRNA control for dPspCas13b-FTO

   Depositor

   Chuan He

   Article

   Wei et al Science. 2022 May 27;376(6596):968-973.

   Insert

   gRNA target sequence for luciferase control
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

   Plasmid

   #190111

   Purpose

   AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Use

   AAV

   Tags

   bpNLS

   Mutation

   H840A

   Promoter

   EF1a

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

   Plasmid

   #221550

   Purpose

   Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human TBK1 (TBK1 Human)
   

   Expression

   Mammalian

   Promoter

   u6

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG001 His6-TwinStrep-SUMO-AapCas12b

   Plasmid

   #153162

   Purpose

   Bacterial expression plasmid for AapCas12b

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

   Insert

   AapCas12b
   

   Expression

   Bacterial

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCAG ERT2-Cre-ERT2 lox

   Plasmid

   #183447

   Purpose

   CreOFF tamoxifen-inducible Cre

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   ERT2-Cre-ERT2
   

   Expression

   Mammalian

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

   Plasmid

   #132405

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA14-pHR-SFFV-GFP-RPA43

   Plasmid

   #199445

   Purpose

   Expression of GFP-RPA43 in mammalian cells

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   RPA43 (POLR1F Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid

    #106964

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG PSAM4-5HT3 HC IRES EGFP

    Plasmid

    #119740

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)
    

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F, R420Q, R424D, R428A

    Promoter

    CAG

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG PSAM4 GlyR IRES EGFP

    Plasmid

    #119739

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
    

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CAG

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid

    #139994

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-MCP-dNS-SRRM1-V5-mCherry

    Plasmid

    #235095

    Purpose

    SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)
    

    Expression

    Mammalian

    Mutation

    The SRRM1 entry clone from DNASU was modified usi…

    Promoter

    CAG

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid

    #103866

    Purpose

    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Myc-GRM1-IRES-CyRFP1

    Plasmid

    #195355

    Purpose

    mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    GRM1-IRES-CyRFP1
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

    Plasmid

    #213728

    Purpose

    Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    bIV spectrin (repeats 13-15) (Sptbn4 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126706

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    

    Use

    CRISPR

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-Akt-FoxO3a-KTR-EGFP

    Plasmid

    #125131

    Purpose

    Fluorescent reporter for Akt activity

    Depositor

    Kazuhiro Aoki

    Article

    Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

    Insert

    FOXO2 (FOXO3 Human)
    

    Tags

    Enhanced green fluorescent protein (EGFP)

    Expression

    Mammalian

    Mutation

    FoxO3A 1-402 amino acid

    Promoter

    CAG promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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