Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,146 results for: pCas

Showing: 2061 - 2080 of 6146 results

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pCA528 KATNA1 MIT residues 1-79

Plasmid

#180614

Purpose

Bacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-79

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNAL1 MIT residues 1-87

Plasmid

#180618

Purpose

Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNAL1 (KATNAL1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-87

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-KATNA1 R14A

Plasmid

#160052

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

R14A mutation

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-KATNA1 V55D

Plasmid

#160053

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

V55D mutation

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-msfGFP-ATG13 (1-197)

Plasmid

#203560

Purpose

Mammalian expression of GFP tagged ATG13 (1-197)

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13 (ATG13 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b-T2A-EGFP

Plasmid

#188271

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-PlmCasX-T2A-EGFP

Plasmid

#188273

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

PlmCasX

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TcCas12b-T2A-EGFP

Plasmid

#188277

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

TcCas12b

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-NAP1 (WT)

Plasmid

#198034

Purpose

Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9-(BB)-2A-Puro-HsHelz-sgRNA_AH

Plasmid

#148897

Purpose

Mammalian Expression of HsHelz-sgRNA

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz-sgRNA (HELZ Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg1HsAGO2_AH

Plasmid

#148848

Purpose

Mammalian Expression of HsAGO2-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsAGO2-sgRNA1 (AGO2 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6A_AH

Plasmid

#148849

Purpose

Mammalian Expression of HsTNRC6A-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsTNRC6A-sgRNA1

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6B_AH

Plasmid

#148850

Purpose

Mammalian Expression of HsTNRC6B-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsTNRC6B-sgRNA1

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg2HsAGO2_AH

Plasmid

#148851

Purpose

Mammalian Expression of HsAGO2-sgRNA2

Depositor

Elisa Izaurralde

Insert

HsAGO2-sgRNA2 (AGO2 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH

Plasmid

#148852

Purpose

Mammalian Expression of HsTNRC6A-sgRNA2

Depositor

Elisa Izaurralde

Insert

HsTNRC6A-sgRNA2

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH

Plasmid

#148853

Purpose

Mammalian Expression of HsTNRC6B-sgRNA3

Depositor

Elisa Izaurralde

Insert

HsTNRC6B-sgRNA3

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

Plasmid

#148854

Purpose

Mammalian Expression of HsAGO1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsAGO1-sgRNA (AGO1 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH

Plasmid

#148856

Purpose

Mammalian Expression of HsNot1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsNot1-sgRNA (CNOT1 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

Plasmid

#148857

Purpose

Mammalian Expression of HsPatL1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsPatL1-sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

Plasmid

#148858

Purpose

Mammalian Expression of HsTNRc6C-sgRNA

Depositor

Elisa Izaurralde

Insert

HsTNRc6C-sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2061 - 2080 of 6146 results

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