We narrowed to 2,368 results for: pcas9

1. pCMV-Target-ACEmax

   Plasmid

   #139105

   Purpose

   Mammalian Target-ACEmax expression vector (pSI-625)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-LAMP1

   Plasmid

   #207787

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9-GFP_sg_mTfeb

   Plasmid

   #79006

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   sgTfeb (Tfeb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. eTd-CBE

   Plasmid

   #196601

   Purpose

   Mammalian expression of eTd-CBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Use

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPapi

   Plasmid

   #96921

   Purpose

   expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.

   Depositor

   David Root, John Doench

   Article

   Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRubiC-T2A-Cas9

   Plasmid

   #75347

   Purpose

   Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   mCherry (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCHKO_HPRT1_exon2_1_Lb

   Plasmid

   #155051

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCHKO_HPRT1_exon3_1_Lb

   Plasmid

   #155053

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX459-TP53-exon4

   Plasmid

   #217455

   Purpose

   For TP53 knockout targeting exon 4 of TP53

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CRISPR Cas9 guide for TP53 (TP53 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-PITCh-CLTC

    Plasmid

    #227312

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CLTC (CLTC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX459-TP53-exon5

    Plasmid

    #217456

    Purpose

    For TP53 knockout targeting exon 5 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. eTd-CBEm

    Plasmid

    #196602

    Purpose

    Mammalian expression of eTd-CBEm

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCPH2A.X_S139A-GS3

    Plasmid

    #204749

    Purpose

    Expression of Cas9 and human H2AX with the S139A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX S139A (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the S139A mutation

    Promoter

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. eTd-CBEa

    Plasmid

    #196603

    Purpose

    Mammalian expression of eTd-CBEa

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpyCas9 PE2*

    Plasmid

    #169850

    Purpose

    Mammalian Expression, SpyCas9 prime editor, optimized NLS

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    spCas9 PE*
    

    Use

    Tags

    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE2-tagRFP-BleoR

    Plasmid

    #192508

    Purpose

    Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

    Depositor

    Gerald Schwank

    Article

    Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    PE2-P2A-tagRFP-P2A-BleoR
    

    Use

    Tags

    BleoR, SV40 bpNLS, and tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. miniCGBE1-NG (pBM1182)

    Plasmid

    #140257

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-RAB7A

    Plasmid

    #227297

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of RAB7A (RAB7A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL11056

    Plasmid

    #68258

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCPH2A.X_S139D-GS3

    Plasmid

    #204748

    Purpose

    Expression of Cas9 and human H2AX with the S139D mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX S139D (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the S139D mutation

    Promoter

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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