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pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A

Plasmid

#169951

Purpose

Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E274R, S297A

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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Ir7a-T2A-QF2 HDR plasmid

Plasmid

#140943

Purpose

Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a gene

Depositor

Leslie Vosshall

Article

Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

Insert

Ir7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK2-A

Plasmid

#138670

Purpose

Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 mouse (Sik2 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK2-B

Plasmid

#138671

Purpose

Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 mouse (Sik2 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd (Y84C/C121S/A139C)

Plasmid

#136063

Purpose

Mammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Y84C/C121S/A139C

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B15-VN

Plasmid

#135514

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*15:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*15:01 (HLA-B Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B57-VN

Plasmid

#135515

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*57:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*57:1 (HLA-B Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B8-VN

Plasmid

#135511

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*08:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*08:01 (HLA-B Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP7.v2 puro

Plasmid

#87795

Purpose

Expresses an inducible short hairpin targeting human MKP7 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP7 (DUSP16 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

Plasmid

#87776

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4 (MAP2K4 Human)

Tags

3xFLAG and NLS

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIB-FLAG-hDIXDC1-L-WT

Plasmid

#61224

Purpose

Retrovirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Retroviral

Tags

FLAG

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-TER+-shDIXDC1-Hu-16

Plasmid

#61237

Purpose

Entry clone encoding shRNA to human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

DIXDC1 (DIXDC1 Human)

Use

Entr clone

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-PGK-Blast-FLAG-hDIXDC1 WT

Plasmid

#61220

Purpose

Lentivirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

PGK

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pBabe-Hygro-FLAG-hDIXDC1 WT

Plasmid

#61213

Purpose

Retrovirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Retroviral

Tags

FLAG

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 WT

Plasmid

#61218

Purpose

Lentivirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV/TO

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-MYC-hDIXDC1-L-F630S

Plasmid

#61241

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

MYC

Mutation

Changed Phenylalanine 630 to Serine

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-MYC-hDIXDC1-L-S592A

Plasmid

#61240

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

MYC

Mutation

Changed Serine 592 to Alanine

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-MYC-hDIXDC1-L-WT

Plasmid

#61239

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

MYC

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-FLAG-hDIXDC1-L-S592A

Plasmid

#61211

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

FLAG

Mutation

Changed Serine 592 to Alanine

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-FLAG-hDIXDC1-L-F630S

Plasmid

#61212

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

FLAG

Mutation

Changed Phenylalanine 630 to Serine

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
AiP14573: pAAV-AiE0452h-minBG-FlpO-WPRE3-BGHpA (Alias: CN4573)

Plasmid

#230871

Purpose

AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

FlpO

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14824: pAAV-AiE0441h_3xC2-minBG-iCre-BGHpA (Alias: CN4824)

Plasmid

#214861

Purpose

Expression of iCre in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15745: pAAV-AiE0452h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5745)

Plasmid

#230880

Purpose

AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mScarlet

Use

AAV

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15138: pAAV-AiE0873m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN5138)

Plasmid

#214868

Purpose

Expression of iCre(R297T) in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre(R297T)

Use

AAV

Mutation

R297T

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13450: pAAV-AiE0140h-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN3450)

Plasmid

#214845

Purpose

Expression of iCre in striatal Pvalb neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13777: pAAV-AiE0682h-minBG-iCre(R297T)-BGHpA (Alias: CN3777)

Plasmid

#214847

Purpose

Expression of iCre(R297T) in striatal Sst neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre(R297T)

Use

AAV

Mutation

R297T

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP15646: pAAV-AiE1426m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5646)

Plasmid

#230884

Purpose

AiE1426m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical and striatal Sst-Chodl neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV2

Insert

SYFP2

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14201: pAAV-AiE0779m_3xC2-minBG-FlpO-WPRE3-BGHpA (Alias: CN4201)

Plasmid

#214855

Purpose

Expression of FlpO in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

FlpO

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14460: pAAV-AiE0452h_3xC2-minBG-SYFP2-WPRE3-BarcodeR-BGHpA (Alias: CN4460)

Plasmid

#236599

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0452h_3xC2 is an enhancer active in striatal D2-MSNs and cortical L5_ET neurons

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12701: pAAV-hChATp-SYFP2-WPRE3-BGHpA (Alias: CN2701)

Plasmid

#236601

Purpose

hChATp is a promoter fragment with activity in striatal cholinergic neurons

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge