We narrowed to 24,999 results for: CHI

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pTS2387-Tier1-PhCMV-p2a_mTagBFP2

Plasmid

#169524

Purpose

Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCHW110035

Plasmid

#222444

Purpose

Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

LoxP-mCherry-stop

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGWB114

Plasmid

#68568

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

Plasmid

#169533

Purpose

Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven ER-localized monomeric red fluorescent protein mRuby2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1

Plasmid

#39280

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF4

Plasmid

#107874

Purpose

Bacterial expression of FGF4-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF4 (FGF4 Synthetic)

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEC3079 (p7INT-Pveg_lacI)

Plasmid

#218505

Purpose

integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

lacI (lacI Escherichia coli)

Expression

Bacterial

Promoter

Pveg

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGWB408

Plasmid

#68673

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pTS2374-Tier1-PhCMV-Gal4_VP16

Plasmid

#169582

Purpose

Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

GAL4-transactivator

Expression

Mammalian

Promoter

PhCMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Sox18

Plasmid

#50782

Purpose

retroviral expression of mouse Sox18

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox18 (Sox18 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pET-NtC60αESNtLC60β

Plasmid

#160878

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, groES

Expression

Bacterial

Promoter

rbcL: T7, chaperones: T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET-NtLNtC60αESC60β

Plasmid

#160875

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL), Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, groES

Expression

Bacterial

Promoter

rbcL: pBAD, chaperones: T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-4xBS_BHLHE40

Plasmid

#153064

Purpose

Four BHLHE40 consensus binding sites cloned upstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

4X consensus BHLHE40 binding sites

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pVH64-Tier1-PSV40-Citrine_p2a_SEAP

Plasmid

#169558

Purpose

Tier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PSV40-driven citrine-P2A-SEAP fusion construct

Expression

Mammalian

Promoter

PSV40

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB215

Plasmid

#68606

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged Citrine (Citri…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(H840A)

Plasmid

#79616

Purpose

Expresses nCas9(H840A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

H840A for nickase Cas9

Promoter

pGal1

Available Since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB402

Plasmid

#68667

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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BB1_12_pGAP

Plasmid

#98498

Purpose

BB1 with insert: promoter PGAP

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

GAP promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB405

Plasmid

#68670

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB131

Plasmid

#68585

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Emerald Luc fused with PEST sequence [ELuc(PEST)]

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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