We narrowed to 2,490 results for: Cre expression vector

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pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176237

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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OTUB1/pRetroX-IRES-DsRed#10

Plasmid

#180279

Purpose

Plasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

human Otubain-1 (OTUB1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_RalA(G23V,full)-P2A-Hygro_Barcode

Plasmid

#170249

Purpose

Barcoded lentiviral vector to express RalA(G23V, full) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

RALA (RALA Human)

Use

Lentiviral

Mutation

G23V

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MAPK9-P2A-Hygro_Barcode

Plasmid

#170238

Purpose

Barcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAPK9 (MAPK9 Human)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb

Plasmid

#141067

Purpose

Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Synthetic Biology

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-EGFP

Plasmid

#118752

Purpose

Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

EGFP

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-ccdb

Plasmid

#118629

Purpose

Multisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

mCherry

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-ccdb

Plasmid

#118628

Purpose

Multisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

EGFP

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-sfGFP+pCI syntron

Plasmid

#172478

Purpose

sfGFP with a synthetic intron from the pCI expression vector (Promega)

Depositor

Mario Capecchi

Insert

sfGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-4

Plasmid

#163130

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons

Plasmid

#172480

Purpose

H2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

Depositor

Mario Capecchi

Insert

H2B-sfGFP-T2A-tdTomato

Use

Cre/Lox

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp7 (SARS-CoV-2)

Plasmid

#169178

Purpose

Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp7 (ORF1ab Synthetic, SARS-CoV-2)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1c_nsp8 (SARS-CoV-2)

Plasmid

#169179

Purpose

Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp8 (ORF1ab Synthetic, SARS-CoV-2)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

Plasmid

#169162

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)

Tags

6His-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

Plasmid

#169163

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-3

Plasmid

#163129

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-2

Plasmid

#163128

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-5

Plasmid

#163131

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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RAP2A-p2A-E2Crimson

Plasmid

#83940

Purpose

Lentiviral vector expressing RAP2A-p2A-E2Crimson

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

p2A-E2Crimson

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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