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pAAV-Neo_CAG-Cas9

Plasmid

#86698

Purpose

Cas9 expression vector for targeting to the AAVS1 locus

Depositor

Ludovic Vallier

Publication

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

pCag

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag-SRm160

Plasmid

#11305

Depositor

Phil Sharp

Publication

Cheng et al Mol Cell Biol. 2006 Jan . 26(1):362-70.

Insert

SRm160 (SRRM1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pQE60-EFTs-His

Plasmid

#124128

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Ts (tsf Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZLCv2-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106357

Purpose

Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

Depositor

Stephen Tapscott

Publication

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3XFLAG and HA

Expression

Mammalian

Promoter

U6

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLentiN

Plasmid

#37444

Purpose

Control lentiviral plasmid containing FLAG-HA tags

Depositor

Karl Munger

Publication

Spangle et al J Virol. 2012 May 2.

Insert

FLAG-HA

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pMSCV mSin1.1 HA

Plasmid

#12582

Depositor

David Sabatini

Publication

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.1 (MAPKAP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

Nov. 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pFA6a-mCherry-hphMX6

Plasmid

#105156

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

mCherry-hphMX6

Tags

mCherry

Expression

Bacterial and Yeast

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Arrb1-RFP

Plasmid

#35401

Depositor

Robert Lefkowitz

Publication

Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

Insert

beta-arrestin-1 (Arrb1 Rat)

Tags

RFP

Expression

Mammalian

Promoter

T7

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
LRP6-pCS2-VSVG

Plasmid

#27282

Depositor

Xi He

Publication

Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

VSVG

Available since

March 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p4028 GST-E6AP

Plasmid

#8655

Depositor

Peter Howley

Publication

Oda et al Proc Natl Acad Sci U S A 1999 Aug 17;96(

Insert

E6-AP (UBE3A Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

Plasmid

#172609

Purpose

Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

2xFLAG-mAID-AMBRA1 (AMBRA1 Synthetic, Human)

Use

CRISPR; Human targeting

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2B-HIF1A prom

Plasmid

#40172

Depositor

Alex Minella

Publication

Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95.

Insert

HIF1A promoter fragment (HIF1A Human)

Use

Luciferase

Expression

Mammalian

Available since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQE30-His-AsnRS

Plasmid

#124105

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Asparaginyl-tRNA ligase (asnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-AspRS-His

Plasmid

#124106

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Aspartate-tRNA ligase (aspS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HA-Daxx/pRK5

Plasmid

#52023

Purpose

Expresses HA-Daxx in mammalian cells

Depositor

Xiaolu Yang

Publication

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

HA-Daxx (DAXX Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX6p1-hPdcd4

Plasmid

#20693

Depositor

Haiwei Song

Publication

Loh et al EMBO J. 2009 Feb 4. 28(3):274-85.

Insert

Programmed Cell Death Protein 4 (PDCD4 Human)

Tags

GST

Expression

Bacterial

Mutation

I36V- does not alter function

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pET21a-ValRS-His

Plasmid

#124122

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Valyl-tRNA ligase (valS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GAK-pmCherryN1

Plasmid

#27695

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

GAK (Gak Mouse)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
MuHKII-pGFPN3

Plasmid

#21922

Depositor

Hossein Ardehali

Publication

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the full length human HKII cDNA sequence …

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GSK3betaS9A (1016)

Plasmid

#49492

Purpose

expresses human Gsk3beta with S9A (constitutively active) mutation

Depositor

Scott Friedman

Publication

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

GSK3beta S9A (GSK3B Human)

Expression

Mammalian

Mutation

S9A (constitutively active)

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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