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We narrowed to 6,946 results for: pcDNA3.1+

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Showing: 2081 - 2100 of 6946 results

  6. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid
    #128746
    Purpose
    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.
    Depositor
    David Bartel
    Publication
    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    Expression
    Mammalian
    Mutation
    5 point mutations disrupting the miR-7 site
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128746-plasmid-map-sequence-id-254125

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  8. pcDNA3.1(+)-ExRai-AKAR-Kras

    Plasmid
    #118408
    Purpose
    Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AKAR-Kras
    Tags
    C-terminal targeting sequence from Kras
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    118408-plasmid-map-sequence-id-242822

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  9. pcDNA3.1-hRIPK1-Flag-K377R

    Plasmid
    #112489
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K377R
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112489-plasmid-map-sequence-id-214536

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  10. pcDNA3.1-hRIPK1-Flag-S320A

    Plasmid
    #112491
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S320A
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112491-plasmid-map-sequence-id-214534

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  15. pcDNA3.1-mouse cdk6 R31C

    Plasmid
    #75171
    Purpose
    Mammalian expression of mouse cdk6 with R31C
    Depositor
    Martine Roussel
    Insert
    cyclin dependent kinase 6 (Cdk6 Mouse)
    Expression
    Mammalian
    Mutation
    R31C
    Promoter
    CMV
    Available since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  17. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

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Showing: 2081 - 2100 of 6946 results