161,799 results

1. M51 Super 8x FOPFlash (TOPFlash mutant)

   Plasmid

   #12457

   Purpose

   Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   mutant TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   mutant TCF/LEF binding sites

   Promoter

   Available since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. [#GS5-RV] MitoTRACER-Donor

   Plasmid

   #233505

   Purpose

   Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

   Insert

   MitoTRACER-Donor
   

   Use

   Retroviral and Synthetic Biology

   Tags

   GFP11 and HA Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

   Plasmid

   #183775

   Purpose

   AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

   Depositor

   Ben Deverman

   Article

   Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

   Insert

   NLS-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   CAG

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. kiCAP-AAV-BI30

   Plasmid

   #183749

   Purpose

   AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.

   Depositor

   Ben Deverman

   Article

   Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

   Insert

   AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   K449R, and NNSTRGG inserted between 588 and 589 o…

   Promoter

   p41

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1-EGFP

   Plasmid

   #19319

   Purpose

   3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3/F-tractin=mStayGold

   Plasmid

   #212019

   Purpose

   For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   F-tractin
   

   Use

   Tags

   mStayGold

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Inducible Caspex expression

   Plasmid

   #97421

   Purpose

   For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

   Depositor

   Steven Carr, Samuel Myers

   Article

   Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

   Inserts

   Caspex
   T2A-Gfp
   

   Use

   CRISPR

   Tags

   3xFLAG and V5

   Expression

   Mammalian

   Mutation

   dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

   Promoter

   TRE3G

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+) eGFP

   Plasmid

   #129020

   Purpose

   Expresses eGFP

   Depositor

   Jeremy Wilusz

   Article

   Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

   Plasmid

   #188768

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SP-dCas9-VPR

    Plasmid

    #63798

    Purpose

    SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    SP-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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