159,557 results

1. pRK5-HA-Ubiquitin-K63

   Plasmid

   #17606

   Purpose

   Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K63 only, other lysines mutated to arginines. Enh…

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGP-CMV-GCaMP6s

   Plasmid

   #40753

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6s
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLJM1-FIRE-pHLy

   Plasmid

   #170775

   Purpose

   Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   CMV

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGGAselect

   Plasmid

   #195714

   Purpose

   pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pSIRV-NF-kB-eCFP

   Plasmid

   #118094

   Purpose

   Flourescent reporter for NF-kB activity

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   eCFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with NF-kB responsive element

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti CMV GFP Puro (658-5)

   Plasmid

   #17448

   Purpose

   3rd gen lentiviral eGFP expression vector, CMV promoter, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Rosa26 mT/mG

   Plasmid

   #17787

   Depositor

   Liqun Luo

   Article

   Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

   Insert

   Rosa26 mT/mG
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   membrane tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PIK3CA - Exon20 H1047R

   Plasmid

   #16639

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Science. 2004 Apr 23. 304(5670):554.

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H1047R

   Promoter

   Available since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK5-HA-Ubiquitin-K27

   Plasmid

   #22902

   Depositor

   Sandra Weller

   Article

   Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K27 only, all other lysines mutated to arginines

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP-alphasynuclein-A53T

    Plasmid

    #40823

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A53T (aggregates more rapidly than the wild-type …

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge