We narrowed to 4,218 results for: Pet2

1. pET28a-PTBP1-RRM34

   Plasmid

   #89156

   Purpose

   Recombinant expression of PTBP1-RRM34 in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
   

   Use

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   contains RNA Recognition Motif 3+4

   Promoter

   T7

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28-a(+)-6xHis-LSSmCherry1(-13)

   Plasmid

   #219415

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Insert

   LSSmCherry1(-13)
   

   Use

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   K137E, K199E

   Promoter

   T7

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaG-42

   Plasmid

   #172758

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-42
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-mCherry-2xEcMTS

   Plasmid

   #133624

   Purpose

   Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

   Insert

   mCherry-2xEcMTS
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-TEV-U1AF37MF77M

   Plasmid

   #168267

   Purpose

   Expresses human dmU1A with the F37M/F77M double mutant

   Depositor

   Joseph Wedekind

   Article

   Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

   Insert

   U1A RRM1 crystallization module (SNRPA Human)
   

   Use

   Tags

   6xHis tag N-terminus, TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   changed F37M and F77M

   Promoter

   T7 promotor

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET23a-His-hRanGAP1tail

   Plasmid

   #53139

   Purpose

   E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   RanGAP1 (RANGAP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C-terminal fragment (amino acids 398-587)

   Promoter

   T7

   Available since

   May 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-Mff(1-61)-PP-GST

   Plasmid

   #73042

   Purpose

   Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

   Depositor

   David Chan

   Article

   Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

   Insert

   Mff(1-61) (Mff Mouse)
   

   Use

   Tags

   GST-tag and PreScission protease site

   Expression

   Bacterial

   Mutation

   Truncation containing amino acids 1-61

   Promoter

   T7

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21b-LaG9(S151C)

   Plasmid

   #172490

   Purpose

   Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   LaG9(S151C)
   

   Use

   Tags

   6xHis, PPX cleavage site, and pelB leader sequenc…

   Expression

   Bacterial

   Mutation

   S151C

   Promoter

   T7

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21-pelB-LaG-6

   Plasmid

   #172741

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-6
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET24a-VHH-2xTS

    Plasmid

    #109419

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge