Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 8,358 results for: BLI;

Showing: 4201 - 4240 of 8358 results

  1. AAV Ef1a-EGFP-WPRE

    Plasmid
    #135428
    Purpose
    Can be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequence
    Depositor
    Rylan Larsen
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135428-plasmid-map-sequence-id-271147

    Enlarge

  2. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid
    #106964
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    106964-plasmid-map-sequence-id-207987

    Enlarge

  3. CBIG-Ngn2

    Plasmid
    #48708
    Purpose
    bicistronic expression of Ngn2 and EGFP
    Depositor
    Jeffrey Macklis
    Insert
    Neurog2 (Neurog2 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/beta-actin
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48708-plasmid-map-sequence-id-70633

    Enlarge

  4. pDEST26-MSRA-C-HA

    Plasmid
    #195177
    Purpose
    Vector for transient expression of MSRA gene with C-terminal HA tag, gene flanked by att sites for gateway cloning
    Depositor
    Stephanie Roessler
    Insert
    MSRA (MSRA Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    195177-plasmid-map-sequence-id-423103

    Enlarge

  5. pCDNA5 FRT TO FLAG LRRK1

    Plasmid
    #232062
    Purpose
    Expresses FLAG-LRRK1 in mammalian cells
    Depositor
    Samara Reck-Peterson
    Insert
    LRRK1 (LRRK1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232062-plasmid-map-sequence-id-461137

    Enlarge

  6. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid
    #121174
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121174-plasmid-map-sequence-id-234993

    Enlarge

  7. pINDUCER20-FLAG-hPAX7 (#734)

    Plasmid
    #78339
    Purpose
    Doxycycline-inducible lentiviral overexpression of human PAX7 ORF with a FLAG tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    PAX7 (PAX7 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. OMM-SPLICSs-nFAST

    Plasmid
    #210430
    Purpose
    Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    OMM-SPLICSs-nFAST
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210430-plasmid-map-sequence-id-415625

    Enlarge

  9. GFP-hChibby1

    Plasmid
    #89472
    Purpose
    Labels mother centrioles/basal bodies
    Depositor
    Ken-Ichi Takemaru
    Insert
    human Chibby1 (CBY1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    89472-plasmid-map-sequence-id-177051

    Enlarge

  10. pHD-DsRed-w+

    Plasmid
    #80926
    Purpose
    Vector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    LoxP-3xP3-DsRed-LoxP
    GMR-white
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80926-plasmid-map-sequence-id-152782

    Enlarge

  11. pRK GFP Paxillin

    Plasmid
    #50529
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    Paxillin (PXN Human)
    Use
    Tags
    GFP21
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50529-plasmid-map-sequence-id-203086

    Enlarge

  12. m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211344
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Monkol Lek
    Insert
    m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211344-plasmid-map-sequence-id-415651

    Enlarge

  13. XLone-BSD SPI1 P2A mCherry

    Plasmid
    #140031
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140031-plasmid-map-sequence-id-275874

    Enlarge

  14. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Use
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. XLone-Puro SPI1 P2A mCherry

    Plasmid
    #140029
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140029-plasmid-map-sequence-id-275844

    Enlarge

  16. pLVXPuro-mGFP-LIC1

    Plasmid
    #66600
    Purpose
    Expresses Dynein-1 LIC1 as a mGFP fusion
    Depositor
    David Stephens
    Insert
    LIC1 (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. 4LG8

    Plasmid
    #60095
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRPF19:JMC069-A01:C226630 (PRPF19 Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Expression
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140032-plasmid-map-sequence-id-275878

    Enlarge

  19. pLV V5-lamin B1

    Plasmid
    #175108
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb1
    Depositor
    Larry Gerace
    Insert
    Lmnb1 (Lmnb1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175108-plasmid-map-sequence-id-351795

    Enlarge

  20. pDEST26-PPP2CB-C-HA

    Plasmid
    #195179
    Purpose
    Vector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloning
    Depositor
    Stephanie Roessler
    Insert
    PPP2CB (PPP2CB Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    195179-plasmid-map-sequence-id-423105

    Enlarge

  21. pcDNA3-myc-Ataxin3Q28

    Plasmid
    #22125
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 21, 2009
    Availability
    Academic Institutions and Nonprofits only

  22. MAP3K6-TAP

    Plasmid
    #69727
    Purpose
    Express tandem tagged MAP3K6 in cells
    Depositor
    Daniel Liebler
    Insert
    Apoptosis signal-regulating kinase 2 (MAP3K6 Human)
    Use
    Tags
    HA-V5 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    69727-plasmid-map-sequence-id-128182

    Enlarge

  23. pLenti-IFT20-EGFP

    Plasmid
    #118032
    Purpose
    Expresses IFT20-EGFP in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human IFT20 (IFT20 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118032-plasmid-map-sequence-id-230381

    Enlarge

  24. pNHA_OROV_Gc

    Plasmid
    #208936
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus Gc protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Gc
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 1579 bp from 5' end, deleted 277 bp …
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208936-plasmid-map-sequence-id-418962

    Enlarge

  25. pDEST-GST-hSYK

    Plasmid
    #211892
    Purpose
    Express human SYK in mammalian cells
    Depositor
    Haian Fu
    Insert
    SYK (SYK Human)
    Use
    Tags
    GST tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    211892-plasmid-map-sequence-id-419133

    Enlarge

  26. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

    Plasmid
    #159920
    Purpose
    Mutagenesis of Vamp2
    Depositor
    Larry Zweifel
    Insert
    Vamp2 (Vamp2 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159920-plasmid-map-sequence-id-317338

    Enlarge

  27. pCDNA alpha5

    Plasmid
    #50500
    Depositor
    Kenneth Yamada
    Insert
    integrin, alpha 5 (ITGA5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pLVXPuro-mGFP-LIC2

    Plasmid
    #66601
    Purpose
    Expresses Dynein-1 LIC2 as a mGFP fusion
    Depositor
    David Stephens
    Insert
    LIC2 (DYNC1LI2 Human)
    Use
    Lentiviral
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. pT7-V5-SBP-C1-HshnRNPK

    Plasmid
    #64923
    Purpose
    Expresses V5- and SBP-tagged hnRNPK in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    hnRNPK (HNRNPK Human)
    Use
    Tags
    V5 and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    64923-plasmid-map-sequence-id-109669

    Enlarge

  30. mEmerald-Enconsin-C-18

    Plasmid
    #62753
    Purpose
    Localization: Microtubule, C1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    Enconsin (MAP7 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62753-plasmid-map-sequence-id-213048

    Enlarge

  31. MINA

    Plasmid
    #39054
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MINA (RIOX2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. pAAV-hSyn-DIO-jGCamp7b-P2A-GSG-mRuby3

    Plasmid
    #130900
    Purpose
    bicistronic plasmid for dual-color neuronal calcium and morphology imaging
    Depositor
    Simon Wiegert
    Insert
    jGCaMP7b-P2A-mRuby3
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130900-plasmid-map-sequence-id-256581

    Enlarge

  33. pCMV-GATA3gfp

    Plasmid
    #83819
    Purpose
    Mammalian expression of GATA3 fused to GFP
    Depositor
    Douglas Engel
    Insert
    GATA3 (Gata3 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83819-plasmid-map-sequence-id-166278

    Enlarge

  34. pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73040
    Purpose
    Express Nluc and copGFP dual reporter in mammalian cells
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK (phosphoglycerate kinase 1)
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73040-plasmid-map-sequence-id-136039

    Enlarge

  35. pBS_flGAD

    Plasmid
    #87031
    Purpose
    Expresses GAD (Glutamic Acid Decarboxylase) in Bacteria
    Depositor
    Janice Blum
    Insert
    flGAD (GAD2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac-Z
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    87031-plasmid-map-sequence-id-173237

    Enlarge

  36. CMV500 A-C/EBPβ

    Plasmid
    #33363
    Depositor
    Charles Vinson
    Insert
    Cebpb (Cebpb Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Dominant Negative (see comments)
    Promoter
    CMV
    Available since
    Dec. 14, 2011
    Availability
    Academic Institutions and Nonprofits only

  37. pYFP-NEAT1pr_v1-RT

    Plasmid
    #97087
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.
    Depositor
    Archa Fox
    Insert
    nuclear paraspeckle assembly transcript 1 (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Expression
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97087-plasmid-map-sequence-id-189642

    Enlarge

  38. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid
    #121170
    Purpose
    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    ABEmax-P2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    P2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121170-plasmid-map-sequence-id-234985

    Enlarge

  39. p3XFlag-S2P

    Plasmid
    #32966
    Purpose
    For mammalian expression of human S2P with a 3x FLAG tag
    Depositor
    Ron Prywes
    Insert
    S2P (MBTPS2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  40. SIRT6

    Plasmid
    #41564
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    aa 3-318
    Promoter
    T7
    Available since
    April 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4201 - 4240 of 8358 results