We narrowed to 13,716 results for: Cas9

1. px335-sgRNA-EF(Cas9n)-mHes1-R

   Plasmid

   #122310

   Purpose

   Expresses sgRNA targeting mouse Hes1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Hes1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. px335-sgRNA-EF(Cas9n)-Peg3-L

   Plasmid

   #122311

   Purpose

   Expresses sgRNA targeting mouse Peg3 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Peg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. px335-sgRNA-EF(Cas9n)-Peg3-R

   Plasmid

   #122312

   Purpose

   Expresses sgRNA targeting mouse Peg3 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Peg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. px335-sgRNA-EF(Cas9n)-Rad21-L

   Plasmid

   #122313

   Purpose

   Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Rad21
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. px335-sgRNA-EF(Cas9n)-Rad21-R

   Plasmid

   #122314

   Purpose

   Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Rad21
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. px335-sgRNA-EF(Cas9n)-Ssrp1-L

   Plasmid

   #122315

   Purpose

   Expresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Ssrp1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. px335-sgRNA-EF(Cas9n)-Ssrp1-R

   Plasmid

   #122316

   Purpose

   Expresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Ssrp1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR-L3-SpCas9-HF4-2A-EGFP-L2

   Plasmid

   #141049

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF4-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR-L3-SpCas9-HF2-2A-EGFP-L2

   Plasmid

   #141050

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF2-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-Himar-dCas9-mammalian

    Plasmid

    #138414

    Purpose

    Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    3xFLAG, 6xHis, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAWp63-clone3-12 (OptiHF-SpCas9)

    Plasmid

    #131737

    Purpose

    Expresses OptiHF-SpCas9 in mammalian cells

    Depositor

    Alan Siu Lun Wong

    Article

    Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

    Insert

    OptiHF-SpCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Q695A+K848A+E923M+T924V+Q926A

    Promoter

    EFS

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT3TS-Hypa-nCas9n-A69

    Plasmid

    #125645

    Purpose

    micro-injection of poly-adenylated Hypa-nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    Hypa-nCas9n
    

    Use

    Rna in vitro transcription vector

    Tags

    Expression

    Mutation

    N692A, M694A, Q695A, H698A from wild-type Cas9

    Promoter

    T3

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFGRNA-LbCpf1-spCas9-MS2

    Plasmid

    #119675

    Purpose

    Empty vector for cloning LbCpf1-spCas9 MS2-fgRNA

    Depositor

    Yongsub Kim

    Article

    Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459V2.0-eSpCas9(1.1)+K855A

    Plasmid

    #108298

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX459V2.0-eSpCas9(1.1)+K810A

    Plasmid

    #108297

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28b-NmCas9-Flag D16A

    Plasmid

    #109031

    Purpose

    D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpCas9 (BB)-2A-GFP gamma-tubulin sg

    Plasmid

    #104437

    Purpose

    Knockdown the expression of gamma-tubulin in human cells

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

    Insert

    gamma-tubulin sgRNA
    

    Use

    Tags

    The gamma-tubulin sgRNA is coexpressed with 3xFLA…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330-Flag-HFSpCas9-H840A

    Plasmid

    #80455

    Purpose

    high fidelity SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    HFSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58694

    Purpose

    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    

    Use

    AAV and CRISPR

    Tags

    GyrA Csplit Intein and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. N-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58693

    Purpose

    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    

    Use

    AAV and CRISPR

    Tags

    3xFlag, GyrA Nsplit Intein, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge