We narrowed to 19,713 results for: ato

1. pcDNA3-ICUE4

   Plasmid

   #181846

   Purpose

   Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   ICUE4 (RAPGEF3 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcrDNA3.1-Chimera

   Plasmid

   #100891

   Purpose

   Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

   Depositor

   Sanford Simon

   Article

   Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

   Insert

   Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pαH-S-GSAS-OMICRON.BA.2-RBD

   Plasmid

   #184830

   Purpose

   Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

   Depositor

   Priyamvada Acharya

   Article

   Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

   Insert

   S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6X His tag

   Expression

   Mammalian

   Mutation

   SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

   Promoter

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pαH-S-GSAS-OMICRON.BA.1-RBD

   Plasmid

   #184831

   Purpose

   Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

   Depositor

   Priyamvada Acharya

   Article

   Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

   Insert

   S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6X His tag

   Expression

   Mammalian

   Mutation

   SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…

   Promoter

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLEX303 TagRFP-T LGALS3 R186S

   Plasmid

   #202428

   Purpose

   Expression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant)

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   TagRFP

   Expression

   Mutation

   R186S

   Promoter

   Available Since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pαH-rS2d

   Plasmid

   #183516

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein S-GSAS-rS2d with (682-685) furin side replaced with GSAS and mutation S383C, D985C

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

   Insert

   rS2d (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFOSGE_COF2F04

   Plasmid

   #70901

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   mod(mdg4) (mod(mdg4) Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCF806-shABC1

   Plasmid

   #186713

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCF806-shABC2

   Plasmid

   #186714

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GCN5 core-dCas9-p300 core

    Plasmid

    #179553

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pαH-rS2d-HexaPro

    Plasmid

    #183515

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

    Plasmid

    #154088

    Purpose

    Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p300 core-dCas9-CBP core

    Plasmid

    #179559

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) & c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV[TetOn]-Puro-TRE3G>mAscl1

    Plasmid

    #198755

    Purpose

    Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

    Depositor

    Nael Nadif Kasri

    Article

    Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pINDUCER-21-RUNX1-P2A-ERG-IMPROVED

    Plasmid

    #154089

    Purpose

    Improved version to express RUNX1, P2A, ERG in mammalian cells.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Runt Related Transcription Factor 1, ETS-Related Gene (RUNX1, ERG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pαH-S-RRAR-OMICRON

    Plasmid

    #180424

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intact

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pαH-S-GSAS-OMICRON.BA.2

    Plasmid

    #184829

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA.2 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pαH-S-GSAS-B.1.617.2.v1

    Plasmid

    #182575

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950N

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GCN5 FL-dCas9-CBP core

    Plasmid

    #179556

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GCN5 core-dCas9-CBP core

    Plasmid

    #179555

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge