We narrowed to 15,116 results for: invs

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pGL3-ACSL4-(-1899)

Plasmid

#202514

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -1899 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pUt-mNF-GFP

Plasmid

#199220

Purpose

LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

hTetR::P2A::EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV D2ir promoter

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-(-706/-277)

Plasmid

#202511

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -277 to -706 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-(-254)

Plasmid

#202512

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -254 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-(-706)

Plasmid

#202513

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -706 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-2

Plasmid

#180398

Purpose

Producing AAV that encodes mouse Irak1 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-2 (Irak1 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-3

Plasmid

#180399

Purpose

Producing AAV that encodes mouse Irak1 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-3 (Irak1 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-1

Plasmid

#180397

Purpose

Producing AAV that encodes mouse Irak1 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-1 (Irak1 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pQE30-VRN1-R249E-R289E-R296E

Plasmid

#159532

Purpose

Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli

Depositor

Joshua Mylne

Article

King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

Insert

VERNALIZATION1 (VRN1 Mustard Weed)

Tags

Six-Histidine tag

Expression

Bacterial

Mutation

Three charge reversal mutations, namely R249E R28…

Promoter

T5

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Nr4a1sgRNA2

Plasmid

#160946

Purpose

Guide RNA 2 to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 gRNA 2 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX304-ARF5-T161A-mNeonGreen

Plasmid

#162026

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Mutation

T161A

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR_P4-P1R:PER36modp

Plasmid

#128431

Purpose

Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).

Depositor

George Haughn

Article

McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

Insert

PEROXISADE36 (AT3G50990 Mustard Weed)

Use

Gateway promoter entry clone

Mutation

Premature stop codons introduced into the Xero1 (…

Promoter

PEROXISADE36

Available Since

Oct. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCDF1-MCS2-EF1-Puro-RGS7-E383K

Plasmid

#113731

Purpose

Express mutant RGS7- E383K- in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

E383K

Promoter

CMV

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-MCS2-EF1-Puro-RGS7-R44C

Plasmid

#113729

Purpose

Express mutant RGS7- R44C- in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666E

Plasmid

#105314

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-646

Plasmid

#92289

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

dGW182 CED 7W7A (gw Fly)

Tags

NHA

Expression

Insect

Mutation

dGW182 CED: aa 940-1215 with the following point …

Promoter

Ac5

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL-PIAL2M-sim1

Plasmid

#67908

Purpose

Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

Depositor

Andreas Bachmair

Article

Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

Insert

PIAL2 (AT5G41580 Mustard Weed)

Tags

MBP

Expression

Bacterial

Mutation

E281-A496; VEDL 425-428 AAA

Promoter

P-lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV6-Entry IL36RN R48W

Plasmid

#58324

Purpose

entry vector for human IL36RN R48W mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Mutation

R48W: c.142C>T, p.Arg48Trp

Promoter

CMV

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pCMV6-Entry IL36RN P76L

Plasmid

#58322

Purpose

entry vector for human IL36RN P76L mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Mutation

P76L: c.227C>T,p.Pro76Leu

Promoter

CMV

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pCMV6-Entry IL36RN Κ35R

Plasmid

#58320

Purpose

entry vector for human IL36RN K35R mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Mutation

Κ35R: c.104A>G, p.Lys35Arg

Promoter

CMV

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only