We narrowed to 7,564 results for: ALP

1. NES-EGFP-PH-BTKx1

   Plasmid

   #183651

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 2-170

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. mRFP-FKBP-INPP5E(D556A)

   Plasmid

   #183678

   Purpose

   Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   INPP5E (INPP5E Human)
   

   Tags

   mRFP-FKBP12(1-108)

   Expression

   Mammalian

   Mutation

   C641A, D556A

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1-nAChr-beta3-CFP

   Plasmid

   #50485

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   CFP fusion in M3-M4 loop (after residue P379). O…

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pBOB-Gpr45-161-3xFlag

   Plasmid

   #245993

   Purpose

   Express 3xFlag C-terminal tagged mouse GPR45-N and GPR161-C fusion protein transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

   Depositor

   Zhao Zhang

   Article

   Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

   Insert

   Gpr45-GPR161161 fusion (Gpr161, Gpr45 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   aa 1 to 327 of Gpr45; aa 357 to 558 of Gpr161

   Promoter

   CMV

   Available Since

   Oct. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV CHMP2B-L4D F5D-d10-52-3xHA (Δα1)

   Plasmid

   #232004

   Purpose

   Expression of a CHMP2B membrane binding mutant (L4D F5D) with the first alpha helix deleted with a 3xHA tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   L4D F5D Δ10-52

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV CHMP2B-L4D F5D-d55-96-3xHA (Δα2)

   Plasmid

   #232005

   Purpose

   Expression of a CHMP2B membrane binding mutant (L4D F5D) with the second alpha helix deleted with a 3xHA tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   L4D F5D Δ55-96

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV CHMP2B-L4D F5D-d97-106-3xHA (Δα2/3 loop)

   Plasmid

   #232006

   Purpose

   Expression of a CHMP2B membrane binding mutant (L4D F5D) with the loop connecting the second and third alpha helices deleted with a 3xHA tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   L4D F5D Δ97-106

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV CHMP2B-L4D F5D-d106-113-3xHA (Δα3)

   Plasmid

   #232007

   Purpose

   Expression of a CHMP2B membrane binding mutant (L4D F5D) with the third alpha helix deleted with a 3xHA tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   L4D F5D Δ106-113

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV CHMP2B-L4D F5D-d118-138-3xHA (Δα4)

   Plasmid

   #232008

   Purpose

   Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fourth alpha helix deleted with a 3xHA tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   L4D F5D Δ118-138

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV CHMP2B-L4D F5D-d159-174-3xHA (Δα5)

    Plasmid

    #232010

    Purpose

    Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fifth alpha helix deleted with a 3xHA tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    L4D F5D Δ159-174

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLViN-iRFP670-α-cateninΔβH

    Plasmid

    #229706

    Purpose

    Lentiviral expression of iRFP670-alpha-catenin-delta-beta-H in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-α-cateninV-

    Plasmid

    #229696

    Purpose

    Transient expression of GFP-alpha-cateninV- in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L344P

    Promoter

    CMV promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xFLAG-DGKδ2-ΔSAMD

    Plasmid

    #226507

    Purpose

    Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene deleted SAM domain

    Depositor

    Fumio Sakane, Chiaki Murakami

    Article

    Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294

    Insert

    DGKD (DGKD Human)
    

    Tags

    Three tandem FLAG epitope tag and enterokinase cl…

    Expression

    Mammalian

    Mutation

    deleted sterile alpha motif domain (deleted amino…

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEF-SpCas9D10A nickase

    Plasmid

    #216736

    Purpose

    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    EF-1-alpha core

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUG36-NES-EGFP-cPHx3

    Plasmid

    #183668

    Purpose

    PtdIns(3,4)P2 biosensor for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Human, Frog)
    

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Yeast

    Mutation

    Amino acids 169-329 (tandem trimer)

    Promoter

    MET25

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUG34-NES-EGFP-cPHx3

    Plasmid

    #183669

    Purpose

    PtdIns(3,4)P2 biosensor for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Human, Frog)
    

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Yeast

    Mutation

    Amino acids 169-329 (tandem trimer)

    Promoter

    MET25

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NES-mCherry-PH-BTKx1

    Plasmid

    #183653

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Human, Frog)
    

    Tags

    X. laevis map2k1.L(32-44)-mCherry

    Expression

    Mammalian

    Mutation

    Amino acids 2-170

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-HIF2α P405A/N847A Δ450-572-pBabe-Puro

    Plasmid

    #25936

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRUPATH Triple (bidirectional) GasS

    Plasmid

    #196055

    Purpose

    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRUPATH Triple Gai1

    Plasmid

    #196048

    Purpose

    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge