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pmCherry-p23

Plasmid

#108224

Purpose

Mammalian expression vector for mCherry fusion to human p23

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

mCherry-p23 (PTGES3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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LMAPCW

Plasmid

#205655

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

mCMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAG463-FKBP-redNFAST

Plasmid

#160364

Purpose

Expresses FKBP-redNFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

FKBP-redNFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG469-LifeAct-redFAST

Plasmid

#160365

Purpose

Expresses LifeAct-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

LifeAct-RedFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBbB9a-GFP

Plasmid

#126112

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDEINO5

Plasmid

#179489

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aacC1

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP713

Plasmid

#45465

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pBbB12a-GFP

Plasmid

#126115

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23151

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG5

Plasmid

#193805

Purpose

Protein production of ChemoG5 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG5

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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mPlum-H2B-6

Plasmid

#55976

Purpose

Localization: Nucleus/Histones, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

H2B (H2BC11 Human)

Tags

mPlum

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pTK_Sox9_enh_349_Citrine

Plasmid

#130594

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_349

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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LMAPPW

Plasmid

#205657

Purpose

shRNAmir backbone for RNA interference, with Puromycin selection

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

mCMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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CDS_mALS

Plasmid

#136081

Purpose

Confers plant resistance to chlorsulfuron

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_mALS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pIM700

Plasmid

#74639

Purpose

alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

none

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T2_AmpR

Plasmid

#119159

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T3_AmpR

Plasmid

#119160

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T1_AmpR

Plasmid

#119158

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Sam68 G178E

Plasmid

#17689

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G178E

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT-ChemoG1

Plasmid

#193806

Purpose

CMV overexpression of ChemoG1 in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG1

Tags

10xHis

Expression

Mammalian

Mutation

EGFP: A206K

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-mTID-HA3-KanMX6

Plasmid

#227982

Purpose

To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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